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. 1979 Dec;83(3):507-12.
doi: 10.1017/s0022172400026358.

A blastogenic test for foot-and-mouth disease

R C Wardley, W G Chapman, A J Garland

A blastogenic test for foot-and-mouth disease

R C Wardley et al. J Hyg (Lond). 1979 Dec.

Abstract

A blastogenic test to detect peripheral blood leukocytes specifically sensitized to foot-and-mouth disease virus antigen is described. The test is carried out in microtitre plates and optimum conditions were found by titration. These employed 7.5 x 10(5) cells/well and 20 complement fixing units of antigen. Peak [3H]thymidine incorporation was found to take place at 2-3 days.

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