Atrial natriuretic peptide and adiponectin interactions in man

Abstract

Reduced circulating natriuretic peptide concentrations are independently associated with insulin resistance and type 2 diabetes, while increased natriuretic peptide levels appear to be protective. Observations in vitro and in heart failure patients suggest that atrial natriuretic peptide (ANP) promotes adiponectin release, an adipokine with insulin sensitizing properties. We tested the hypothesis that ANP acutely raises adiponectin levels in 12 healthy men. We infused ANP intravenously over 135 minutes while collecting venous blood and adipose tissue microdialysates at baseline and at the end of ANP-infusion. We obtained blood samples at identical time-points without ANP infusion in 7 age and BMI matched men. With infusion, venous ANP concentrations increased ∼10 fold. Systemic and adipose tissue glycerol concentrations increased 70% and 80%, respectively (P<0.01). ANP infusion increased total adiponectin 14 ± 5% and high molecular-weight (HMW)-adiponectin 13 ± 5% (P<0.05). Adiponectin did not change in the control group (P<0.05 vs. infusion). ANP-induced changes in HMW adiponectin and adipose tissue lipolysis were directly correlated with each other, possibly suggesting a common mechanism. Our data show that ANP acutely increases systemic total and HMW-adiponectin concentrations in healthy subjects. Our study could have implications for the physiological regulation of adiponectin and for disease states associated with altered natriuretic peptide availability.

Figure 1. ANP induces total and HMW-adiponectin.

A – Individual changes in venous total as well as HMW-adiponectin with ANP infusion. The bar indicates the mean value (P = compared to baseline, n = 12). B – Correlation between the change in plasma ANP and the change in HMW adiponectin with ANP infusion (n = 11). C – Individual changes in dialysate glycerol in subcutaneous adipose tissue (SCAT) with ANP infusion. The bar indicates the mean value (P = compared to baseline, n = 11). D - Correlation between Δ SCAT dialysate glycerol, a marker for lipolysis, and the change in HMW adiponectin with ANP infusion (n = 11).