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. 2012:2012:421683.
doi: 10.1155/2012/421683. Epub 2012 Aug 5.

Isolation and characterization of chitosan-producing bacteria from beaches of chennai, India

Kuldeep Kaur  1 , Vikrant Dattajirao, Vikas Shrivastava, Uma Bhardwaj
Affiliations

Isolation and characterization of chitosan-producing bacteria from beaches of chennai, India

Kuldeep Kaur et al. Enzyme Res. 2012.

Abstract

Chitosan is a deacetylated product of chitin produced by chitin deacetylase, an enzyme that hydrolyses acetamido groups of N-acetylglucosamine in chitin. Chitosan is a natural polymer that has great potential in biotechnology and in the biomedical and pharmaceutical industries. Commercially, it is produced from chitin via a harsh thermochemical process that shares most of the disadvantages of a multistep chemical procedure. It is environmentally unsafe and not easily controlled, leading to a broad and heterogeneous range of products. An alternative or complementary procedure exploiting the enzymatic deacetylation of chitin could potentially be employed, especially when a controlled and well-defined process is required. In this study, 20 strains of bacteria were isolated from soil samples collected from different beaches of Chennai, India. Of these 20 bacterial strains, only 2 strains (S3, S14) are potent degrader of chitin and they are also a good producer of the enzyme chitin deacetylase so as to release chitosan.

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Figures

Figure 1
Figure 1
Figure 2
Figure 2
Chitinolytic activity was studied using chitin agar.
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