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. 2012:2012:904763.
doi: 10.1155/2012/904763. Epub 2012 Jul 15.

Experimental Investigations on the Effects of Carbon and Nitrogen Sources on Concomitant Amylase and Polygalacturonase Production by Trichoderma viride BITRS-1001 in Submerged Fermentation

Arotupin Daniel Juwon  1 , Ogunmolu Funso Emmanuel
Affiliations

Experimental Investigations on the Effects of Carbon and Nitrogen Sources on Concomitant Amylase and Polygalacturonase Production by Trichoderma viride BITRS-1001 in Submerged Fermentation

Arotupin Daniel Juwon et al. Biotechnol Res Int. 2012.

Abstract

The paper investigates the effects of different commercial carbon and nitrogen sources on the concomitant synthesis of amylase and polygalacturonase enzymes with the aim of optimizing them for maximal enzyme production. The microorganism used in this work was the fungus Trichoderma viride BITRS-1001, which had been previously identified as a highly active producer of amylase and polygalacturonase enzymes. The results showed that the different commercial carbon and nitrogen substrate significantly affected the concomitant syntheses of amylase and polygalacturonase in culture media supplemented with the different commercial carbon and nitrogen substrates. The result obtained suggested that for optimal and concomitant synthesis of the enzymes by Trichoderma viride BITRS-1001 in submerged fermentation, minimal medium supplemented with maltose and casein were the carbon and nitrogen substrates of choice.

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Figures

Figure 1
Figure 1
Effect of different carbon sources on the biomass of T. viride BITRS-1001 in submerged culture (SmC).
Figure 2
Figure 2
pH variations in the culture media during fermentation in submerged culture (SmC).
Figure 3
Figure 3
Effect of different carbon sources on amylase activity of T. viride BITRS-1001 in submerged culture (SmC).
Figure 4
Figure 4
Effect of different carbon sources on polygalacturonase activity of T. viride BITRS-1001 in submerged culture (SmC).
Figure 5
Figure 5
Effect of different nitrogen sources on the biomass of T. viride BITRS-1001 in submerged culture (SmC).
Figure 6
Figure 6
pH variations in the culture media during fermentation of T. viride BITRS-1001 in submerged culture (SmC).
Figure 7
Figure 7
Effect of different nitrogen sources on amylase activity of T. viride BITRS-1001 in submerged culture (SmC).
Figure 8
Figure 8
Effect of different nitrogen sources on polygalacturonase activity of T. viride BITRS-1001 in submerged culture (SmC).
Figure 9
Figure 9
Relationship between changes in the pH of the culture media with amylase activity of T. viride BITRS-1001 in casein supplemented medium.
Figure 10
Figure 10
Relationship between changes in the pH of the culture media with polygalacturonase activity of T. viride BITRS-1001 in casein supplemented medium.
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