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. 1990 Dec;61(6):350-3.
doi: 10.1007/BF01738548.

Rapid detection of chimeric bcr/abl mRNAs in acute lymphoblastic and chronic myeloid leukemia by the polymerase chain reaction

J Maurer  1 , E Thiel
Affiliations

Rapid detection of chimeric bcr/abl mRNAs in acute lymphoblastic and chronic myeloid leukemia by the polymerase chain reaction

J Maurer et al. Blut. 1990 Dec.

Abstract

We have developed a rapid method for the detection of bcr/abl mRNAs, the products of the BCR/ABL fusion genes. The method is based on the polymerase-chain-reaction (PCR). Through the use of additional internal primers it is possible to detect directly a single Ph1-positive cell among 10(5) unaffected cells thus omitting time-consuming blotting procedures. The whole analytical procedure starting from RNA isolation to agarose gel electrophoresis including two rounds of PCR can be performed in less than six hours.

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