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. 1979 Dec;32(3):1028-32.
doi: 10.1128/JVI.32.3.1028-1032.1979.

Heteroduplex analysis of the RNA of clone 3 Moloney murine sarcoma virus

Y H Chien, I M Verma, P H Duesberg, N Davidson

Heteroduplex analysis of the RNA of clone 3 Moloney murine sarcoma virus

Y H Chien et al. J Virol. 1979 Dec.

Abstract

Heteroduplex analysis of the RNA isolated from purified virions of clone 3 Moloney murine sarcoma virus (M-MSV) hybridized to cDNA's from Moloney murine leukemia virus (M-MLV) and clone 124 M-MSV shows that the main physical component of clone 3 RNA is missing all or most of the 1.5-kilobase (kb) clone 124 M-MSV specific sequence denoted beta s (S. Hu et al. Cell 10:469--477, 1977). This sequence is either deleted in clone 3 RNA or substituted by a very short (0.3-kilobase) sequence. In other respects, clone 3 and clone 124 RNAs show the same heteroduplex structure relative to M-MLV. Since beta s is believed to contain the src gene(s) of clone 124 RNA, this result leaves as an unresolved question the nature of the src gene(s) of the clone 3 M-MSV RNA complex.

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References

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