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. 1990 Oct;108(4):673-6.
doi: 10.1093/oxfordjournals.jbchem.a123261.

Purification and characterization of soluble human IL-6 receptor expressed in CHO cells

K Yasukawa  1 , T Saito, T Fukunaga, Y Sekimori, Y Koishihara, H Fukui, Y Ohsugi, T Matsuda, H Yawata, T Hirano, et al.
Affiliations
Free article

Purification and characterization of soluble human IL-6 receptor expressed in CHO cells

K Yasukawa et al. J Biochem. 1990 Oct.
Free article

Abstract

An immunosorbent assay system to detect genetically engineered IL-6 receptor (IL-6R) was established, whereby soluble IL-6 receptor (sIL-6R) was detected in the culture medium when sIL-6R cDNA was transfected into COS1 cells. A stably transformed Chinese hamster ovary (CHO) cell line constitutively expressing sIL-6R has been established. The recombinant sIL-6R was purified to homogeneity by sequential filtration and chromatography of the culture medium. The recombinant sIL-6R augmented the sensitivity of M1 cells to IL-6 in growth inhibition assay in a dose-dependent manner. Furthermore, a radioisotope immunosorbent assay (RIA) utilizing recombinant sIL-6R was established which could detect IL-6 in a quantity as low as 10 ng/ml.

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