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. 2012 Aug 28:11:298.
doi: 10.1186/1475-2875-11-298.

Genetic variation in histidine rich proteins among Indian Plasmodium falciparum population: possible cause of variable sensitivity of malaria rapid diagnostic tests

Navin Kumar  1 , Jai Pn Singh, Veena Pande, Neelima Mishra, Bina Srivastava, Ridhima Kapoor, Neena Valecha, Anupkumar R Anvikar
Affiliations

Genetic variation in histidine rich proteins among Indian Plasmodium falciparum population: possible cause of variable sensitivity of malaria rapid diagnostic tests

Navin Kumar et al. Malar J. .

Abstract

Background: Rapid diagnostic tests (RDTs) have revolutionized the diagnosis of malaria. Among the various factors affecting RDTs sensitivity is genetic variation of the antigen used. The genetic variation in PfHRP2 and PfHRP3 proteins was studied among the Indian Plasmodium falciparum isolates.

Methods: One hundred and forty isolates of P. falciparum were collected from six geographical regions of India. Target genes encoding PfHRP2 and PfHRP3 antigens were sequenced to study genetic polymorphism. Minimum detection limit giving a positive rapid diagnostic test was also determined.

Results: Extensive variations were observed in amino acid repeat types of PfHRP2 and PfHRP3. PfHRP2 exhibited more polymorphism than PfHRP3. Significant relation was observed between type 2 and type 7 repeats and RDT detection rate as higher number of these repeats showed better sensitivity with RDTs.

Conclusion: The results provide insights into the genetic diversity of Pfhrp2 and Pfhrp3 genes among Indian P. falciparum population and its relation to RDT sensitivity.

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Figures

Figure 1
Figure 1
Map of India showing geographical distribution of samples (140 P. falciparum isolates).1. Chhattisgarh (n = 40), 2. Gujarat (n = 20), 3. Karnataka (n = 10), 4. Madhya Pradesh (n = 20), 5. Orissa (n = 30), 6. West Bengal (n = 20).
Figure 2
Figure 2
Relationship between number of type 2 × type 7 amino acid repeats of PfHRP2 and average minimum detection limit of RDTs among six different geographical regions of India.
See this image and copyright information in PMC

References

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