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. 2012 Aug 29;17(9):10344-54.
doi: 10.3390/molecules170910344.

Preparation of dry extract of Mikania glomerata Sprengel (Guaco) and determination of its coumarin levels by spectrophotometry and HPLC-UV

Luciana Soares E Soares e Silva  1 , Luciane Santos da Santos da Silva, Larissa Brumano, Paulo César Stringheta, Miriam Aparecida de Oliveira Pinto, Leticia Oliveira Moreira Dias, Camila de Sá Martins Muller, Elita Scio, Rodrigo Luiz Fabri, Helena C Castro, Maria da Penha Henriques do Amaral
Affiliations

Preparation of dry extract of Mikania glomerata Sprengel (Guaco) and determination of its coumarin levels by spectrophotometry and HPLC-UV

Luciana Soares E Soares e Silva et al. Molecules. .

Abstract

Guaco (Mikania glomerata Sprengel) syrup is one of the most popular herbal medicines used to treat the symptoms of asthmatic bronchitis, cough and hoarseness. The coumarin 2H-1-benzopyran-2-one, is one of the major constituents of Guaco and contributes to its pharmacological effects. The pharmaceutical capsule form of dry extract of Guaco is recommended by the Brazilian Program of Medicinal Plants and Herbal Medicines and used in primary health care. In order to identify a new protocol to obtain the raw material for Guaco capsule production we evaluated two methods, including a freezedrying process (lyophilization) and the spray-dryer technique, as well as the use of two adjuvants, Maltodextrins and Aerosil®, in different concentrations. The coumarin levels of the dried extracts were analyzed by UV-spectrophotometry and HPLC-UV/DAD. The adjuvant Aerosil® 8% showed better dry powder physical appearance. Lyophilization was observed to be the best process to obtain the dry extract of Guaco based on the measured coumarin levels.

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Figures

Figure 1
Figure 1
Guaco lyophilized (A and B) and atomized (C and D) extracts using maltodextrin (A and C) or Aerosil® (B and D) as adjuvant, respectively.
Figure 2
Figure 2
Adhesion of the product in the solid-gas separator and collection system of the spray dryer equipment (Model Büchi Mini Spray Dryer B-191, Büchi Labortechnik AG, Flawil, Switzerland).
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