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. 2012 Mar;53(3):299-302.

Open-drop anesthesia for small laboratory animals

Tara E Risling  1 , Nigel A Caulkett, Darrel Florence
Affiliations

Open-drop anesthesia for small laboratory animals

Tara E Risling et al. Can Vet J. 2012 Mar.

Abstract
in English, French

This study examined the effect of temperature on volatile concentrations of 2 inhalant anesthetics, isoflurane (ISO) and sevoflurane (SEVO), delivered via open-drop technique, as well as the characteristics of induction and recovery using the open-drop method in mice. Testing revealed that temperature had no effect on the volatile concentration of either ISO or SEVO. However, it was determined that open-drop delivery of ISO or SEVO is a viable means of anesthetizing mice under certain conditions. The volatile concentration required to induce anesthesia in mice following the application of 0.5 mL of anesthetic in an induction chamber of 725 mL volume at 87.6 kPa and 20°C was measured with a precision gas analyzer. For ISO, anesthesia was induced at concentrations of 6.80 ± 0.57% [mean ± standard deviation (s)] after 35.70 ± 6.95 s (n = 10), while SEVO induction took significantly longer (45.50 ± 9.96 s) and required higher volatile concentrations [7.41 ± 0.57% (n = 10)]. The animals recovered rapidly from both ISO and SEVO-based induction.

Anesthésie par méthode ouverte pour les petits animaux de laboratoire. Cette étude a examiné l’effet de la température sur les concentrations volatiles de 2 anesthésiques par inhalation, l’isoflurane (ISO) et le sévoflurane (SEVO), administrés à l’aide d’une méthode ouverte ainsi que les caractéristiques de l’induction et du réveil en utilisant la méthode ouverte chez les souris. Les essais ont révélé que la température n’avait aucun effet sur la concentration volatile de l’ISO ou du SEVO. Cependant, il a été déterminé que l’administration par méthode ouverte de l’ISO ou du SEVO était une méthode viable d’anesthésier les souris dans certaines conditions. La concentration volatile requise pour induire l’anesthésie chez les souris après l’application de 0,5 ml d’anesthésique dans une chambre d’induction d’un volume de 725 ml à 87,6 kPa et à 20 °C a été mesurée à l’aide d’un analyseur de gaz de précision. Pour l’ISO, l’anesthésie a été induite à des concentrations de 6,80 ± 0,57 % (moyenne ± déviation standard) après 35,70 ± 6,95 s (n = 10), tandis que l’induction à l’aide du SEVO a exigé un délai significativement plus long (45,50 ± 9,96 s) et a requis des concentrations volatiles supérieures (7,41 ± 0,57 % [n = 10]). Les animaux se sont réveillés rapidement de l’induction à base d’ISO et de SEVO.

(Traduit par Isabelle Vallières)

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Figures

Figure 1
Figure 1
Volatile concentration of sevoflurane and isoflurane in an anesthetic induction chamber as delivered via open drop technique (n = 8). The volatile concentration response curves differed significantly between the two drugs (F1, 20 = 1.824, P < 0.05).
See this image and copyright information in PMC

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