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. 2012;13(7):8775-8788.
doi: 10.3390/ijms13078775. Epub 2012 Jul 16.

Microwave-assisted method for simultaneous extraction and hydrolysis for determination of flavonol glycosides in Ginkgo foliage using Brönsted acidic ionic-liquid [HO(3)S(CH(2))(4)mim]HSO(4) aqueous solutions

Huanhuan Yao  1 , Xinxuan Du  1 , Lei Yang  1 , Wenjie Wang  1 , Fengjian Yang  1 , Chunjian Zhao  1 , Xiangdong Meng  1 , Lin Zhang  1 , Yuangang Zu  1
Affiliations

Microwave-assisted method for simultaneous extraction and hydrolysis for determination of flavonol glycosides in Ginkgo foliage using Brönsted acidic ionic-liquid [HO(3)S(CH(2))(4)mim]HSO(4) aqueous solutions

Huanhuan Yao et al. Int J Mol Sci. 2012.

Abstract

The Brönsted acidic ionic-liquid [HO(3)S(CH(2))(4)mim] HSO(4), a novel dual catalyst-solvent, has been successfully applied in simultaneous microwave-assisted extraction and hydrolysis for the determination of flavonol glycosides in Ginkgo foliage. The parameters, namely the [HO(3)S(CH(2))(4)mim]HSO(4) concentration, microwave-irradiation power, microwave-irradiation time, and solid-liquid ratio, were optimized. The optimum conditions were: an amount of 1.5 M [HO(3)S(CH(2))(4)mim]HSO(4), a microwave-irradiation power of 120 W, an irradiation time of 15 min, and a solid-liquid ratio of 1:30 g/mL. Compared with traditional methods the proposed approach demonstrates higher efficiency in a shorter operating time, and is an efficient, rapid, and simple sample preparation method.

Keywords: Ginkgo biloba; [HO3S(CH2)4mim]HSO4; flavonol glycoside; hydrolysis; ionic liquid; microwave-assisted extraction.

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Figures

Figure 1
Figure 1
Effects of [HO3S(CH2)4mim]HSO4 concentration: (a) microwave power, (b) microwave time, (c) solid–liquid ratio and (d) ionic-liquid-based microwave-assisted simultaneous extraction and hydrolysis (ILMASEH) process was performed in a microwave unit with a power of 700 W. Dried sample was mixed with [HO3S(CH2)4mim]HSO4 in water at different concentrations and then irradiated with microwaves for specified times.
Figure 2
Figure 2
Comparative HPLC chromatograms for quercetin, kaempferol, and isorhamnetin in an extract obtained using ILMASEH (1.5 M [HO3S(CH2)4mim]HSO4 as dual catalyst–solvent) (a) and using EPM method (b). Inset: chemical structures of three predominant flavonol aglycones in Ginkgo biloba.
Figure 3
Figure 3
Schematic diagram of ionic liquids based microwave-assisted simultaneous extraction and hydrolysis apparatus.
See this image and copyright information in PMC

References

    1. Kleijnen J., Knipschild P. Ginkgo biloba. Lancet. 1992;340:1136–1139. - PubMed
    1. Kudolo G.B., Dorsey S., Blodgett J. Effect of the ingestion of Ginkgo biloba extract on platelet aggregation and urinary prostanoid excretion in healthy and Type 2 diabetic subjects. Thromb. Res. 2002;108:151–160. - PubMed
    1. Guidetti C., Paracchini S., Lucchini S., Cambieri M., Marzatico F. Prevention of neuronal cell damage induced by oxidative stress in vitro: Effect of different Ginkgo biloba extracts. J. Pharm. Pharm. 2001;53:387–392. - PubMed
    1. Van Beek T.A. Ginkgolides and bilobalide: Their physical, chromatographic and spectroscopic properties. Bioorg. Med. Chem. 2005;13:5001–5012. - PubMed
    1. Ahlemeyer B., Krieglstein J. Neuroprotective effects of Ginkgo biloba extract. Cell. Mol. Life Sci. 2003;60:1779–1792. - PMC - PubMed

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