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. 2012;13(7):8805-8818.
doi: 10.3390/ijms13078805. Epub 2012 Jul 16.

Isolation and characterization of cross-amplification microsatellite panels for species of Procapra (Bovidae; Antilopinae)

Jing Chen  1   2 , Chunlin Li  1   2   3 , Ji Yang  1   2 , Zhenhua Luo  1   2 , Songhua Tang  1 , Feng Li  1   2 , Chunwang Li  1 , Bingwan Liu  4 , Zhigang Jiang  1   2
Affiliations

Isolation and characterization of cross-amplification microsatellite panels for species of Procapra (Bovidae; Antilopinae)

Jing Chen et al. Int J Mol Sci. 2012.

Abstract

The three Procapra species, Tibetan gazelle (P. picticaudata), Mongolian gazelle (P. gutturosa) and Przewalski's gazelle (P. przewalskii) are endemic to Asia. Several intraspecific genetic issues have been studied with species-specific microsatellite loci in these Asian gazelles. However, cross-species microsatellite panels are absent, which inhibits comparative conservation and evolutionary studies of the Procapra. In this study, we isolated 20 cross-species microsatellite loci for Procapra from both related species and the genomic library of P. przewalskii. Fifty-three samples of the three gazelles were used to characterize the markers. Allele numbers ranged from three to 20, with a mean of 7.93 per locus. Observed heterozygosity (H(O)) averaged 0.680 and expected heterozygosity (H(E)) 0.767. The mean polymorphic information content (PIC) was 0.757 for P. picticaudata, 0.803 for P. gutturosa and 0.590 for P. przewalskii. Nine loci were significantly deviated from Hardy-Weinberg (H-W) equilibrium in the three species. Significant linkage disequilibrium was detected in four pairs of loci in P. przewalskii, five pairs in P. gutturosa and 51 pairs in P. picticaudata. Considering the abundance of published loci and their high success rates of cross-amplification, testing and utilization of loci from related species is efficient for wild species of Bovidae. The cross-species microsatellite loci we developed will facilitate further interspecies genetic studies in Procapra.

Keywords: Procapra; cross-amplification; genetic markers; isolation strategy; microsatellite.

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Figures

Figure 1
Figure 1
Map of the distribution ranges and sampling locations of Procapra (P. picticaudata, P. gutturosa and P. przewalskii).
See this image and copyright information in PMC

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