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. 2012 Oct;22(10):1502-4.
doi: 10.1038/cr.2012.127. Epub 2012 Sep 4.

Recognition of methylated DNA by TAL effectors

Dong Deng, Ping Yin, Chuangye Yan, Xiaojing Pan, Xinqi Gong, Shiqian Qi, Tian Xie, Magdy Mahfouz, Jian-Kang Zhu, Nieng Yan, Yigong Shi

Recognition of methylated DNA by TAL effectors

Dong Deng et al. Cell Res. 2012 Oct.
No abstract available

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Figures

Figure 1
Figure 1
The TALE repeats containing code NG, but not HD, recognize 5-methylcytosine. (A) The crystal structure of DNA-bound dHax3 TALE repeats (PDB accession code 3V6T). RVDs in each repeat are shown as red spheres. The upper and lower DNA strands are colored gold and silver. (B) Structural analysis of the code NG→T suggested that NG may recognize 5-methycytosine (mC). (C) The DNA fragments with three bases T substituted with mC retained binding to TALE repeats. The protocol of EMSA is described in detail in Supplementary information, Data S1. (D) Crystal structure of dHax3 in complex with methylated DNA dHax3-5mC at 1.85 Å. The overall structure is identical to Figure 1A; see also Supplementary information, Figure S2A. Only one mC and the Gly13 in the corresponding repeat is shown to highlight the van der Waals interaction, which is indicated by the black dashed line, between the 5-methyl group of mC and the Cα atom of Gly. (E) Crystal structure of a dHax3 variant in complex with methylated DNA containing (mC)G(mC)G at 1.95 Å resolution. The hydrogen bond between Asn13 and base G is shown as red dashed line. The Cα atoms of Gly13 residues are shown as red spheres. (F) NG, but not HD, recognizes 5-methylcytosine. Lanes 1-20: The DNA of dHax3 box with six bases of T replaced by mC retained binding to dHax3 repeats, whereas replacement by C led to complete loss of binding. Lanes 21-40: HD specifically recognizes C. Substitution of the five cytosines in dHax3 box with any other nucleotides, including mC, leads to almost complete loss of binding with dHax3 repeats. All the structure figures were prepared with PyMOL.
See this image and copyright information in PMC

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