Effects of ischemia and reperfusion on protein synthesis in livers with different glutathione levels

Abstract

The role of oxygen-free radicals for metabolic derangements in the ischemic and reperfused liver is controversial. The effect on hepatic protein synthesis of a 60-minute period of ischemia followed by two hours of reperfusion was studied in four groups of rats with different hepatic contents of the oxygen free radical scavenger glutathione (GSH): group 1, fed rats; group 2, fed rats treated with diethylmaleate (DEM) one hour before use (0.69 mL/kg, i.p.); group 3, 48-hour fasted rats; and group 4, 48-hour fasted rats treated with cobalt-chloride (45 mg/kg, s.c.) ten hours before use. Protein synthesis rates were determined by measuring incorporation of U-14C-leucine into protein in incubated liver slices. Treatment of fed rats with DEM and fasting for 48 hours significantly reduced liver GSH content. The effect of fasting on liver GSH was reversed by treatment with cobalt-chloride. The protein synthesis rate was reduced to approximately 30% of initial value at the end of the ischemic period and recovered to 70% to 100% of initial value after two hours of reperfusion with no differences between the experimental groups. Thus the effect of liver ischemia and reperfusion on protein synthesis was similar in groups of rats with different hepatic GSH contents at the onset of ischemia. The data suggest that oxygen free radicals do not play a major role for the impairment of protein synthesis in the ischemic and reperfused liver.