Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2012 Oct;43(10):2800-2.
doi: 10.1161/STROKEAHA.112.669465. Epub 2012 Sep 4.

Effect of focal ischemia on long noncoding RNAs

Ashutosh Dharap  1 , Venkata Prasuja Nakka, Raghu Vemuganti
Affiliations

Effect of focal ischemia on long noncoding RNAs

Ashutosh Dharap et al. Stroke. 2012 Oct.

Abstract

Background and purpose: Long noncoding RNAs (lncRNAs) play a significant role in cellular physiology. We evaluated the effect of focal ischemia on the expression of 8314 lncRNAs in rat cerebral cortex using microarrays.

Methods: Ischemia was induced by transient middle cerebral artery occlusion. Genomic and transcriptomic correlates of the stroke-responsive lncRNAs and the transcription factor binding sties in their promoters were evaluated with bioinformatics.

Results: Three hundred fifty-nine lncRNAs were upregulated (>2-fold) and 84 were downregulated (<0.5-fold) at 3 hours to 12 hours of reperfusion after middle cerebral artery occlusion compared with sham. Sixty-two stroke-responsive lncRNAs showed >90% sequence homology with exons of protein-coding genes. Promoters of stroke-responsive lncRNA genes and their homologous protein-coding genes showed highly overlapping transcription factor binding sites. Despite presence of open reading frames, lncRNAs did not form any product when subjected to in vitro translation.

Conclusions: Stroke significantly alters cerebral lncRNA expression profiles.

PubMed Disclaimer

Conflict of interest statement

Conflict of Interest: None

References

    1. Cheng J, Kapranov P, Drenkow J, Dike S, Brubaker S, Patel S, et al. Transcriptional maps of 10 human chromosomes at 5-nucleotide resolution. Science. 2005;308:1149–1154. - PubMed
    1. Dharap A, Bowen K, Place R, Li LC, Vemuganti R. Transient focal ischemia induces extensive temporal changes in rat cerebral micrornaome. J Cereb Blood Flow Metab. 2009;29:675–687. - PMC - PubMed
    1. Dharap A, Nakka VP, Vemuganti R. Altered expression of piwi rna in the rat brain after transient focal ischemia. Stroke. 2011;42:1105–1109. - PMC - PubMed
    1. Rinn JL, Chang HY. Genome regulation by long noncoding rnas. Annu Rev Biochem. 2012;81:145–166. - PMC - PubMed
    1. Faghihi MA, Modarresi F, Khalil AM, Wood DE, Sahagan BG, Morgan TE, et al. Expression of a noncoding rna is elevated in Alzheimer's disease and drives rapid feed-forward regulation of beta-secretase. Nat Med. 2008;14:723–730. - PMC - PubMed

Publication types

Substances