Hypervariable minisatellite DNA is a hotspot for homologous recombination in human cells
- PMID: 2295091
- DOI: 10.1016/0092-8674(90)90719-u
Hypervariable minisatellite DNA is a hotspot for homologous recombination in human cells
Abstract
Hypervariable minisatellite DNA sequences are short tandemly repeated sequences that are present throughout the human genome and are implicated to enhance recombination. We have constructed a consensus hypervariable minisatellite sequence and analyzed its effect on homologous recombination in human cells in culture. The consensus sequence d(AGAGGTGGGCAGGTGG)6.5 is shown to stimulate homologous recombination up to 13.5-fold. The stimulation occurs at a distance and in both directions but does show a quantitative directionality. Stimulation occurs in a codominant manner, and the sequence is inherited equally in the products. Enhancement is maintained, but at a reduced level, when double-strand breaks are introduced into the substrates. Multiple unselected recombination events are promoted, and preferential stimulation of reciprocal exchange events is demonstrated.
Similar articles
-
A non-hypervariable human minisatellite strongly stimulates in vitro intramolecular homologous recombination.J Mol Biol. 1998 May 8;278(3):499-505. doi: 10.1006/jmbi.1998.1714. J Mol Biol. 1998. PMID: 9600833
-
Recombination hotspot activity of hypervariable minisatellite DNA requires minisatellite DNA binding proteins.Somat Cell Mol Genet. 1998 Jan;24(1):41-51. doi: 10.1007/BF02677494. Somat Cell Mol Genet. 1998. PMID: 9776980 Free PMC article.
-
Hypervariable 'minisatellite' regions in human DNA.Nature. 1985 Mar 7-13;314(6006):67-73. doi: 10.1038/314067a0. Nature. 1985. PMID: 3856104
-
Minisatellite variant repeat mapping: application to DNA typing and mutation analysis.EXS. 1993;67:125-39. doi: 10.1007/978-3-0348-8583-6_12. EXS. 1993. PMID: 8400685 Review.
-
Molecular arrangement and evolution of heterochromatic DNA.Annu Rev Genet. 1980;14:121-44. doi: 10.1146/annurev.ge.14.120180.001005. Annu Rev Genet. 1980. PMID: 6260016 Review. No abstract available.
Cited by
-
Characterization of Sp1, AP-1, CBF and KRC binding sites and minisatellite DNA as functional elements of the metastasis-associated mts1/S100A4 gene intronic enhancer.Nucleic Acids Res. 2001 Aug 15;29(16):3335-46. doi: 10.1093/nar/29.16.3335. Nucleic Acids Res. 2001. PMID: 11504871 Free PMC article.
-
The extent, mechanism, and consequences of genetic variation, for recombination rate.Am J Hum Genet. 1996 Dec;59(6):1175-83. Am J Hum Genet. 1996. PMID: 8940261 Free PMC article. No abstract available.
-
Characterization of a low copy repetitive element S232 involved in the generation of frequent deletions of the distal short arm of the human X chromosome.Nucleic Acids Res. 1992 Mar 11;20(5):1117-22. doi: 10.1093/nar/20.5.1117. Nucleic Acids Res. 1992. PMID: 1549475 Free PMC article.
-
Nonrandom distribution of chloroplast recombination events in Chlamydomonas reinhardtii: evidence for a hotspot and an adjacent cold region.Genetics. 1992 Oct;132(2):413-29. doi: 10.1093/genetics/132.2.413. Genetics. 1992. PMID: 1358751 Free PMC article.
-
Evolutionary consequences of nonrandom damage and repair of chromatin domains.J Mol Evol. 1992 Aug;35(2):156-80. doi: 10.1007/BF00183227. J Mol Evol. 1992. PMID: 1501255 Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
