Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1990 Jan;87(1):439-43.
doi: 10.1073/pnas.87.1.439.

Expression of human adenosine deaminase in mice reconstituted with retrovirus-transduced hematopoietic stem cells

J M Wilson  1 , O Danos, M Grossman, D H Raulet, R C Mulligan
Affiliations

Expression of human adenosine deaminase in mice reconstituted with retrovirus-transduced hematopoietic stem cells

J M Wilson et al. Proc Natl Acad Sci U S A. 1990 Jan.

Abstract

Recombinant retroviruses encoding human adenosine deaminase (ADA; adenosine aminohydrolase, EC 3.5.4.4) have been used to infect murine hematopoietic stem cells. In bone marrow transplant recipients reconstituted with the genetically modified cells, human ADA was detected in peripheral blood mononuclear cells of the recipients for at least 6 months after transplantation. In animals analyzed in detail 4 months after transplantation, human ADA and proviral sequences were detected in all hematopoietic lineages; in several cases, human ADA activity exceeded the endogenous activity. These studies demonstrate the feasibility of introducing a functional human ADA gene into hematopoietic stem cells and obtaining expression in multiple hematopoietic lineages long after transplantation. This approach should be helpful in designing effective gene therapies for severe combined immunodeficiency syndromes in humans.

PubMed Disclaimer

References

    1. Mol Cell Biol. 1987 Feb;7(2):838-46 - PubMed
    1. Biochem Biophys Res Commun. 1974 Apr 8;57(3):590-5 - PubMed
    1. Mol Cell Biol. 1988 Dec;8(12):5116-25 - PubMed
    1. J Mol Biol. 1975 Nov 5;98(3):503-17 - PubMed
    1. J Biol Chem. 1976 Sep 25;251(18):5448-56 - PubMed

Publication types