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. 2010 Sep;1(5):885-888.
doi: 10.3892/ol_00000157. Epub 2010 Sep 1.

Synovial sarcoma individual chemotherapy directed by collagen gel droplet embedded culture drug sensitivity test: A case report

Feng Gu  1 , Yongjie Ma, Yu Fan, Ronggang Lang, Ting Ding, Xishan Hao, Yun Gong, Hisayuki Kobayahsi, Zhiwei Fang, Li Fu
Affiliations

Synovial sarcoma individual chemotherapy directed by collagen gel droplet embedded culture drug sensitivity test: A case report

Feng Gu et al. Oncol Lett. 2010 Sep.

Abstract

The collagen gel droplet embedded culture-drug sensitivity test (CD-DST) is an anticancer drug sensitivity test developed about 10 years ago. This study reports the application of this test in the choice of neoadjuvant chemotherapy for the treatment of one patient with a large synovial sarcoma in the right shank. A 28-year old man presented at our hospital with a large mass in his right shank which had a hard texture and an obscure boundary. The histopathological diagnosis of excisional biopsy specimens was synovial sarcoma with low differentiation. Theprubicin/Cisplatin (THP/CDDP) neoadjuvant chemotherapy was selected based on the results obtained from the CD-DST. After three courses, a computed tomography (CT) scan was performed which indicated that the volume of the tumor had decreased significantly. Additionally, tumor necrosis, as well as the clinical response, showed complete response. The histopathological diagnosis of wide excision specimens indicated a grade III chemotherapy response. The patient was alive and without recurrence after a follow-up of 16 months. Our results indicated that the CD-DST is a useful tool for selecting neoadjuvant chemotherapeutic drugs for patients with synovial sarcoma.

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Figures

Figure 1
Figure 1
A large synovial sarcoma in the right shank. (A) Prior to and (B) after neoadjuvant chemotherapy and local tumor excision.
Figure 2
Figure 2
MRI of synovial sarcoma in the right shank prior to neoadjuvant chemotherapy. The sarcoma is 15×10×9 cm, with an obscure boundary and uneven texture and without any destruction of the bone. (A) Axial plane. (B) Sagital plane.
Figure 3
Figure 3
(A) H&E staining of excisional biopsy specimens prior to neoadjuvant chemotherapy (magnification, ×200). (B) H&E staining of local tumor excision specimens after neoadjuvant chemotherapy. Fibroblast cell proliferation and tumor cell necrosis, grade III chemotherapy response (magnification, ×200).
Figure 4
Figure 4
The survival rate of tumor cells and results of the CD-DST. CDDP and THP have a significant effect on the growth of tumor cells, while VCR and 5-FU show no significant effects. IFO was used as a negative control. Based on these results, CDDP/THP neoadjuvant chemotherapy was selected.
Figure 5
Figure 5
A CT scan of a synovial sarcoma in the right shank after neoadjuvant chemotherapy. (A) A precontrast scan showed that the volume of the sarcoma had decreased to 11×8.2 cm, and (B) a contrast scan revealed tumor necrosis.
See this image and copyright information in PMC

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