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. 1990 Jan 19;247(4940):318-20.
doi: 10.1126/science.2296720.

Prenylated proteins: the structure of the isoprenoid group

H C Rilling  1 , E Breunger, W W Epstein, P F Crain
Affiliations

Prenylated proteins: the structure of the isoprenoid group

H C Rilling et al. Science. .

Abstract

The mevalonate-derived portion of a prenylated protein from Chinese hamster ovary cells has been established as diterpenoid (C20). This group is linked to a carboxyl-terminal cysteine as a thioether. It was removed from the protein by hydrazinolysis followed by Raney nickel desulfurization, and the resulting hydrocarbon fraction was analyzed by gas chromatography-mass spectrometry.

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