The mammalian gene function resource: the International Knockout Mouse Consortium

Abstract

In 2007, the International Knockout Mouse Consortium (IKMC) made the ambitious promise to generate mutations in virtually every protein-coding gene of the mouse genome in a concerted worldwide action. Now, 5 years later, the IKMC members have developed high-throughput gene trapping and, in particular, gene-targeting pipelines and generated more than 17,400 mutant murine embryonic stem (ES) cell clones and more than 1,700 mutant mouse strains, most of them conditional. A common IKMC web portal (www.knockoutmouse.org) has been established, allowing easy access to this unparalleled biological resource. The IKMC materials considerably enhance functional gene annotation of the mammalian genome and will have a major impact on future biomedical research.

Fig. 1

Vectors used by the IKMC: targeting vectors: a EUCOMM/KOMP-CSD knockout-first allele; b KOMP-Regeneron null allele generating large deletions; c NorCOMM promoter-driven targeting vector. Most commonly used trapping vectors: d conditional EUCOMM vector rsFlipROSAβgeo*; e TIGM vector VICTR76; f NorCOMM vector UPA