Rapid detection of carbapenemase-producing Pseudomonas spp

Abstract

A novel biochemical technique, the Carba NP test, has been evaluated to detect carbapenemase production in Pseudomonas spp. This test was specific (100%), sensitive (94.4%), and rapid (<2 h). This cost-effective test, which could be implemented in any microbiology laboratory, offers a reliable technique for identification of carbapenemase-producing Pseudomonas spp.

Fig 1

Examples of results for non-carbapenemase-producing and carbapenemase-producing Pseudomonas spp. from the Carba NP test. Non-carbapenemase producers are as follows: P. aeruginosa 76110, wild type, P. aeruginosa PU 21, wild type; P. aeruginosa ATCC 27853, wild type; P. aeruginosa PAO1, wild type; P. aeruginosa HT29, OprM porin deficient; P. aeruginosa PAO1, OprD porin deficient; P. aeruginosa PAO1, overexpressing MexC/D-OprM efflux pump; P. aeruginosa, overexpressing MexA/B-OprM efflux pump; P. aeruginosa PAO1, overexpressing MexX/Y-OprM efflux pump; P. aeruginosa 3-12, overexpressing the chromosomal AmpC; P. aeruginosa, GES-1; P. aeruginosa, GES-9; P. aeruginosa ED, OXA-28; P. aeruginosa, OXA-32; P. aeruginosa, SHV-2a; P. aeruginosa, SHV-5; P. aeruginosa, PER-1; P. aeruginosa, VEB-1; and P. aeruginosa, BEL-1 (rows 1 to 19, respectively). Carbapenemase producers are as follows; P. aeruginosa 16, SPM-1; P. stutzeri, DIM-1; P. aeruginosa P13, KPC-2; P. aeruginosa PA-2, KPC-2; P. aeruginosa, VIM-1; Pseudomonas fluorescens COU, VIM-2; P. aeruginosa REZ, VIM-2; P. putida, VIM-2; P. stutzeri, VIM-2; P. aeruginosa CAS, VIM-4; P. aeruginosa JAC, VIM-4; P. aeruginosa 1287, IMP-1; P. stutzeri PB207, IMP-1; P. putida NTU 92/99, IMP-1; P. aeruginosa 0607097, IMP-2; P. aeruginosa, IMP-13; P. aeruginosa 453, NDM-1; P. aeruginosa 353, NDM-1; P. aeruginosa 73-56, GIM-1; P. aeruginosa WCH2677, AIM-1; P. fluorescens, BIC-1; P. aeruginosa GW-1, GES-2; and P. aeruginosa, GES-5 (rows 20 to 42, respectively). Note that strains in rows 41 and 42 gave false-negative results.