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. 2001 Jan;8(1):59-62.

Plasmid-mediated streptomycin resistance of listeria monocytogenes

E Purwati  1 , S Radu, Z Hassan, O W Ling, R A Rahim
Affiliations

Plasmid-mediated streptomycin resistance of listeria monocytogenes

E Purwati et al. Malays J Med Sci. 2001 Jan.

Abstract

A strain of streptomycin-resistant Listeria monocytogenes LM35 isolated from imported frozen beef was examined in this study. In conjugation studies, the L. monocytogenes LM35 strain harbouring two plasmids of 54, 3.0, 2.8 and 2.7 kilobase was used as the donor and streptomycin-sensitive and plasmidless L. monocytogenes LM65 and LM100 strains as the recipients. Streptomycin resistance was transferred to L. monocytogenes LM65 and LM100 strains at frequencies of 3.3 × 10(-8) and 1.2 × 10(-9) per input donor cells, respectively. In both occasions, we also observed the concomitant transfer of the donor's 54 kilobase plasmid. These results suggest that streptomycin resistance in L. monocytogenes LM35 was mediated by the 54 kilobase plasmid.

Keywords: Listeria monocytogenes; plasmid; streptomycin; transfer.

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Figures

Figure 1:
Figure 1:
Agarose gel (0.85%) gel electrophoresis of plasmid DNA from L. monocytogenes strains and their respective transconjugants. Lanes: 1, transconjugant LM65; 2, transconjugant LM100; 3 and 4, donor L35; M, E.
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