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. 2011 May;2(3):545-550.
doi: 10.3892/etm.2011.233. Epub 2011 Mar 21.

Anti-inflammatory effect of patchouli alcohol isolated from Pogostemonis Herba in LPS-stimulated RAW264.7 macrophages

Yan-Fang Xian  1 , Yu-Cui Li, Siu-Po Ip, Zhi-Xiu Lin, Xiao-Ping Lai, Zi-Ren Su
Affiliations

Anti-inflammatory effect of patchouli alcohol isolated from Pogostemonis Herba in LPS-stimulated RAW264.7 macrophages

Yan-Fang Xian et al. Exp Ther Med. 2011 May.

Abstract

Pogostemonis Herba has long been used in traditional Chinese medicine for the treatment of inflammation-related disorders. Patchouli alcohol (PA) isolated from Pogostemonis Herba is a tricyclic sesquiterpene that is known to exert a variety of pharmacological activities. The present study aimed to investigate the anti-inflammatory effect of PA on lipopolysaccharide (LPS)-stimulated RAW264.7 cells. Pre-treatment with PA at concentrations of 10, 20 or 40 μM dose-dependently decreased the production of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, nitric oxide (NO) and prostaglandin E(2) in LPS-stimulated RAW264.7 cells. In addition, PA treatment also reversed the increased mRNA expression of TNF-α, IL-1β, IL-6, inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 caused by LPS in RAW264.7 cells. These results indicate that PA is an important anti-inflammatory constituent of Pogostemonis Herba and that its anti-inflammatory effect may be mediated, at least in part, by down-regulation of the mRNA expression of a panel of inflammatory mediators, such as TNF-α, IL-1β, IL-6, iNOS and COX-2.

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Figures

Figure 1.
Figure 1.
Chemical structure of patchouli alcohol.
Figure 2.
Figure 2.
Effects of PA on the cell viability of RAW264.7 cells. The values shown represent the mean ± SEM (n=6).
Figure 3.
Figure 3.
Effects of PA on the production of NO (A) and mRNA expression of iNOS (B) in LPS-stimulated RAW264.7 cells. The values shown represent the mean ± SEM (n=3–6). #p<0.01 compared to the control group; *p<0.05, **p<0.01 and ***p<0.01 compared to the LPS group.
Figure 4.
Figure 4.
Effects of PA on the production of PGE2 (A) and mRNA expression of COX-2 (B) in LPS-stimulated RAW264.7 cells. The values shown represent the mean ± SEM (n=3–6). #p<0.01 compared to the control group; *p<0.05 and **p<0.01 compared to the LPS group.
Figure 5.
Figure 5.
Effects of PA on the production of TNF-α (A), IL-6 (B) and IL-1β (C) in LPS-stimulated RAW264.7 cells. The values shown represent the mean ± SEM (n=6). #p<0.01 compared to the control group; *p<0.05 and **p<0.01 compared to the LPS group.
Figure 5.
Figure 5.
Effects of PA on the production of TNF-α (A), IL-6 (B) and IL-1β (C) in LPS-stimulated RAW264.7 cells. The values shown represent the mean ± SEM (n=6). #p<0.01 compared to the control group; *p<0.05 and **p<0.01 compared to the LPS group.
Figure 6.
Figure 6.
Effects of PA on the mRNA expression of TNF-α (A), IL-6 (B) and IL-1β (C) in LPS-stimulated RAW264.7 cells. The values shown represent the mean ± SEM (n=3). #p<0.01 compared to the control group; *p<0.05 and **p<0.01 compared to the LPS group.
Figure 6.
Figure 6.
Effects of PA on the mRNA expression of TNF-α (A), IL-6 (B) and IL-1β (C) in LPS-stimulated RAW264.7 cells. The values shown represent the mean ± SEM (n=3). #p<0.01 compared to the control group; *p<0.05 and **p<0.01 compared to the LPS group.
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