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. 2012 Jan 1;8(26):6871-6875.
doi: 10.1039/c2sm25693b. Epub 2012 May 31.

Biomimetic Fabrication of Genetically-Engineered Collagen Peptide-Assembled Freestanding Films Reinforced by Quantum Dot Joints

Zengyan Wei  1 , Yoshiaki Maeda, Hiroshi Matsui
Affiliations

Biomimetic Fabrication of Genetically-Engineered Collagen Peptide-Assembled Freestanding Films Reinforced by Quantum Dot Joints

Zengyan Wei et al. Soft Matter. .

Abstract

Genetically-engineered collagen peptides were assembled into freestanding films when QDs are co-assembled as joints between collagen domains. These peptide based films show excellent mechanical properties with Young's modulus of ~20 GPa, much larger than most of multi-composite polymer films and previously reported freestanding nanoparticle-assembled sheets, and it is even close to the bone tissue in nature. These films show little permanent deformation under small indentation while the mechanical hysteresis becomes remarkable when the load approaches near and beyond the rupture point, which is also characteristic to the bone tissue.

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Figures

Figure 1
Figure 1
Images of the collagen peptide-QD freestanding films on holey substrates. (a) AFM image and height profiles (between arrows) of the intact peptide-QD film. (b) AFM image of a broken part of the peptide-QD film. (c) TEM image of the intact peptide-QD film. (d) Magnified TEM image of the rectangular area highlighted in (c). (e) TEM image of the broken peptide-QD film. (f) Magnified TEM image of the rectangular area highlighted in (e).
Figure 2
Figure 2
TEM images for control experiments. (a–c) Casting the solution containing B877B peptides and carboxyl QDs instead of streptavidin-QDs on holey Cu grids. The magnified TEM iamge of a square area in (b) was shown in (c). (d–e) Casting the solution containing B877B peptides and streptavidin-QDs on holey Cu grids by drying slowly in 70–90% humidity at 4°C.
Figure 3
Figure 3
(a) A typical force (F)-displacement (δ) curve of the freestanding peptide-QD film. (b) The rescaled loading curve (highlighted in the rectangular area of Figure 1(a) and a fitting with Eq. 1 (blue line). The curve approaches cubic behavior at high loads (inset). (c–d) Histogram of film pretensions and Young’s modulus of the freestanding peptide-QD film, respectively. Dashed lines in (c) and (d) represent Gaussian fits. (e) A typical F - δ curve of the freestanding peptide-QD film on large scale of nanoindentation. (f) Rescaled loading curve highlighted in the rectangular area of Figure 3(e). The rupture point is marked by an arrow. Frup = 340 nN, and δrup = 52 nm.
Scheme 1
Scheme 1
Schematic representation of the formation of peptide-QD freestanding films.
See this image and copyright information in PMC

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