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. 2013:927:175-86.
doi: 10.1007/978-1-62703-038-0_16.

Flow cytometric methods for sperm assessment

Vanesa Robles  1 , Felipe Martínez-Pastor
Affiliations

Flow cytometric methods for sperm assessment

Vanesa Robles et al. Methods Mol Biol. 2013.

Abstract

Flow cytometry allows the assessment of multiple sperm parameters following a diverse number of protocols. Here, we describe three methods for evaluating critical aspects of sperm quality: the double stain SYBR-14 and propidium iodide (PI) for assessing sperm viability; the double stain PNA-FITC (peanut agglutinin-fluorescein isothiocyanate) and PI for assessing acrosomal integrity combined with sperm viability; and JC-1 (5,5',6,6'-tetrachloro-1,1',3,3'-tetraethyl-benzimidazolylcarbocyanine iodide) for assessing mitochondrial membrane potential. These three stains are widely used and can be analyzed with basic flow cytometers.

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