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. 2012 Dec;97(12):4650-5.
doi: 10.1210/jc.2012-1440. Epub 2012 Sep 19.

Serum anti-müllerian hormone levels in healthy females: a nomogram ranging from infancy to adulthood

S Lie Fong  1 , J A Visser, C K Welt, Y B de Rijke, M J C Eijkemans, F J Broekmans, E M Roes, W H M Peters, A C S Hokken-Koelega, B C J M Fauser, A P N Themmen, F H de Jong, I Schipper, J S E Laven
Affiliations

Serum anti-müllerian hormone levels in healthy females: a nomogram ranging from infancy to adulthood

S Lie Fong et al. J Clin Endocrinol Metab. 2012 Dec.

Abstract

Context: Anti-müllerian hormone (AMH) is an accurate marker of ovarian reserve. However, sufficiently large sets of normative data from infancy to the end of reproductive life are scarce.

Objective: This study was an assessment of serum AMH levels in healthy females.

Subjects: In 804 healthy females ranging from infancy until the end of the reproductive period, serum AMH levels were measured with an enzyme-linked immunometric assay. All adults had regular menstrual cycles. The majority was proven fertile and none of them had used oral contraceptive pills prior to study inclusion.

Results: In the total cohort, AMH was inversely correlated with age (r = -0.24; P < 0.001). The age at which the maximum AMH value was attained was at 15.8 yr. In girls younger than 15.8 yr, serum AMH and age were positively correlated (r = +0.18; P = 0.007). Thereafter AMH levels remained stable (r = -0.33; P = 0.66), whereas from the age of 25.0 yr onward, an inverse correlation between AMH and age (r = -0.47; P < 0.001) was observed. At any given age, considerable interindividual differences in serum AMH levels were observed.

Conclusion: During infancy AMH levels increase, whereas during adolescence, a plateau until the age of 25 yr was observed. From the age of 25 yr onward, serum AMH levels correlate inversely with age, implying that AMH is applicable as a marker of ovarian reserve only in women of 25 yr old and older. Our nomogram may facilitate counseling women on their reproductive potential.

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Figures

Fig. 1.
Fig. 1.
AMH nomogram based on natural linear spline interpolation. Reference lines of serum AMH for the 10th, 50th, and 90th percentiles of predicted AMH values vs. age.
Fig. 2.
Fig. 2.
Serum AMH levels (micrograms per liter) per subgroup based on age. Reference lines of serum AMH for the 10th, 50th, and 90th percentiles of predicted AMH values vs. age in subgroup 0–15.8 yr (left panel), 16–25 yr (middle panel), and in women older than 25 yr (right panel).
See this image and copyright information in PMC

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