Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2013 Jan;164(1):1-5.
doi: 10.1016/j.resmic.2012.09.004. Epub 2012 Sep 17.

Natural lysogenization and transduction in Salmonella enterica serovar Choleraesuis by bacteriophage P1

Marcela León  1 , Javier Santander, Roy Curtiss 3rd, James Robeson
Affiliations

Natural lysogenization and transduction in Salmonella enterica serovar Choleraesuis by bacteriophage P1

Marcela León et al. Res Microbiol. 2013 Jan.

Abstract

It has been reported that bacteriophage P1 injects DNA into serovar Choleraesuis without evidence of productive infection. However, we found that P1 generates progeny and is capable of transduction in serovar Choleraesuis. This is not the case with other serovars of Salmonella enterica we tested. Therefore, P1 could play a role in serovar Choleraesuis evolution and contribute to its genetic manipulation and analysis.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
A. Spots tests of P1 lysogens. 1 and 3 correspond to two different Cmr derivates of serovar Choleraesuis χ3246 obtained by infection with P1cml,clr100; 5 corresponds to E. coli χ1912T; 2 and 4 correspond to a duplicate (two independent colonies) of wild-type serovar Choleraesuis χ3246; 6 corresponds to E. coli C χ1912C. B. LPS profile; C. PCR verification of traJ gene (500 bp) present in pLM2, outer lanes are 1 kb and 100 bp DNA ladder respectively; D. Plasmid profile and antibiotic patterns of the bacterial strains indicated.
See this image and copyright information in PMC

References

    1. Goldberg RB, Bender RA, Streicher SL. Direct selection for P1-sensitive mutants of enteric bacteria. J Bacteriol. 1974;118:810–814. - PMC - PubMed
    1. Kaiser D, Dworkin M. Gene transfer to a Myxobacterium by Escherichia coli phage P1. Science. 1975;187:653–654. - PubMed
    1. Kelly SM, Bosecker BA, Curtiss R., III Characterization and protective properties of attenuated mutants of Salmonella choleraesuis. Infect Immunol. 1992;60:4881–4890. - PMC - PubMed
    1. LeMinor L. The genus Salmonella. In: Balows AH, Trüper H, Dworkin M, Harder W, Schleifer KH, editors. The Prokaryotes A Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications. second. III. Springer-Verlag; New York: 1991.
    1. Lennox ES. Transduction of linked genetic characters of the host by bacteriophage P1. Virology. 1955;1:199–206. - PubMed

Publication types

MeSH terms

LinkOut - more resources