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. 2012 Jul;14(7):408-16.
Epub 2012 Jul 30.

Genotypic Analysis of E. coli Strains Isolated from Patients with Cystitis and Pyelonephritis

M Anvarinejad  1 , Sh Farshad, R Ranjbar, G M Giammanco, A Alborzi, A Japoni
Affiliations

Genotypic Analysis of E. coli Strains Isolated from Patients with Cystitis and Pyelonephritis

M Anvarinejad et al. Iran Red Crescent Med J. 2012 Jul.

Abstract

Background: Urinary tract infection is the most common health problem affecting millions of people each year, mainly caused by a large genetically heterogeneous group of Escherichia coli called uropathogenic E. coli This study investigates the genotypic analysis of E. coli strains isolated from patients with cystitis and pyelonephritis.

Methods: During 2008-2009, 90 E. coli strains were analyzed, consisting of 48 isolates causing pyelonephritis in children and 42 isolates causing cystitis. Having identified the strains by standard methods, they were subtyped by pulsed field gel electrophoresis (PFGE) and their corresponding patterns were compared using dendrogram.

Results: Sixty five PFGE profiles were obtained from the genome of E. coli strains by this genotyping method. Thirty six and thirty three patterns were obtained for pyelonephritis and cystitis, respectively. Most strains exhi-bited twelve and thirteen bands and the patterns with eight or nineteen bands had the lowest rate. Genome sizes of the strains were between 1610-4170 kbp.

Conclusion: With due attention to these results, genetic patterns showed that the strains had different clonalities and it could be suggested in some cases that the strains causing pyelonephritis or cystitis have common patterns and different diseases could be explained by different gene factors.

Keywords: Cystitis; Electrophoresis; Genetic patterns; Pyelonephritis; Uropathogenic Escherichia coli.

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Conflict of interest statement

Conflict of Interest: None declared.

Figures

Fig. 1
Fig. 1
A dendrogram showing relationships among E. coli isolates from children with pyelonephritis (P) and cystitis (C).
Fig. 2
Fig. 2
A dendrogram showing relationships among E. coli isolates from children with pyelonephritis (P).
Fig. 3
Fig. 3
A dendrogram showing relationships among E. coli isolates from children with cystitis (C).
See this image and copyright information in PMC

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