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. 2013 Jan;144(1):56-58.e7.
doi: 10.1053/j.gastro.2012.09.017. Epub 2012 Sep 19.

Replication of hepatitis C virus genotype 3a in cultured cells

Mohsan Saeed  1 , Claire Gondeau, Susu Hmwe, Hiroshi Yokokawa, Tomoko Date, Tetsuro Suzuki, Takanobu Kato, Patrick Maurel, Takaji Wakita
Affiliations

Replication of hepatitis C virus genotype 3a in cultured cells

Mohsan Saeed et al. Gastroenterology. 2013 Jan.

Abstract

Hepatitis C virus (HCV) genotype 3a is widespread worldwide, but no replication system exists for its study. We describe a subgenomic replicon system for HCV genotype 3a. We determined the consensus sequence of an HCV genome isolated from a patient, and constructed a subgenomic replicon using this clone. The replicon was transfected into HuH-7 cells and RNA replication was confirmed. We identified cell culture-adaptive mutations that increased colony formation multiple-fold. We have therefore established a genotype 3a replicon system that can be used to study this HCV genotype.

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Comment in

  • Hepatitis C virus replicons volume 3 and 4.
    Lohmann V, Bartenschlager R. Lohmann V, et al. Gastroenterology. 2013 Jan;144(1):13-5. doi: 10.1053/j.gastro.2012.11.013. Epub 2012 Nov 16. Gastroenterology. 2013. PMID: 23164572 No abstract available.

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