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. 2012 Jul;12(7):437-41.
doi: 10.5812/hepatmon.6133. Epub 2012 Jul 30.

Phylogenetic analysis of torque teno virus in hepatitis C virus infected patients in shiraz

Azra Kenar Koohi  1 , Mehrdad Ravanshad, Manouchehr Rasouli, Shahab Falahi, Ashraf Baghban
Affiliations

Phylogenetic analysis of torque teno virus in hepatitis C virus infected patients in shiraz

Azra Kenar Koohi et al. Hepat Mon. 2012 Jul.

Abstract

Background: Torque teno virus (TTV) was the first human Circoviridae detected in a Japanese patient with unknown hepatitis in 1997. Subsequently, several studies performed to evaluate different aspects of Torque teno virus pathogenesis.

Objectives: The present study aimed to determine dominant genotype of Torque teno virus in chronic hepatitis disease using 5΄-UTR sequence among patients infected by hepatitis C virus in Shiraz - Iran.

Patients and methods: The study conducted in 240 patients with chronic hepatitis C from Prof. Alborzi Clinical Microbiology Research Center. The presence of Torque teno virus DNA and its genotype in plasma was assessed by nested polymerase chain reaction using two primer sets for 5΄-UTR and N22 regions. Phylogenetic analysis was performed based on 5΄-UTR region.

Results: DNA of Torque teno virus was detected in 220 out of 240 (92 %) patients with chronic hepatitis C by the use of 5΄-UTR primer based PCR method and in 12 out of 240 (5%) by the use of N22 primer. Based on phylogenetic analysis it was shown that the Dominant genotype in this study was 11. Genotypes 1, 3, 17, and 22 were also detected. Some sequences could not be classified to a specific genotype.

Conclusions: The prevalence of Torque teno virus DNA in patients with chronic hepatitis C disease by the use of 5΄-UTR primer appeared to be higher compared to that revealed by N22 primer. We observed five genotypes among hepatitis C chronic patients in our study.

Keywords: Hepatitis C; Prevalence; Shiraz.

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Figures

Figure 1
Figure 1. Constructed Phylogenetic Tree by the Use of Kimura Two-Parameter Matrix and Neighbor-Joining Method, Based on 5’-UTR Sequence (220nt- residues3- 233).
References: 5’-UTR sequence from TTV genomes were identified by their GenBank accession numbers
Figure 2
Figure 2. Constructed Phylogenetic Tree by the Use of Maximum Parsimony Method, Based on 5’-UTR Sequence (220nt-residues3- 233)
References: 5’-UTR Sequence from TTV genomes were identified by their GenBank Accession Numbers
See this image and copyright information in PMC

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