Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2012 Jul-Sep;80(3):581-90.
doi: 10.3797/scipharm.1203-07. Epub 2012 Apr 30.

Simultaneous Estimation of Amlodipine Besylate and Indapamide in a Pharmaceutical Formulation by a High Performance Liquid Chromatographic (RP-HPLC) Method

Deval B Patel  1 , Falgun A Mehta, Kashyap K Bhatt
Affiliations

Simultaneous Estimation of Amlodipine Besylate and Indapamide in a Pharmaceutical Formulation by a High Performance Liquid Chromatographic (RP-HPLC) Method

Deval B Patel et al. Sci Pharm. 2012 Jul-Sep.

Abstract

An isocratic reversed-phase liquid chromatograpic assay method was developed for the quantitative determination of amlodipine besylate (AML) and indapamide (IND) in combined dosage form. A Brownlee C-18, 5 μm column with a mobile phase containing 0.02 M potassium dihydrogen phosphate-methanol (30+70, v/v) total pH-adjusted to 3 using o-phosphoric acid was used. The flow rate was 1.0 mL min(-1) and effluents were monitored at 242 nm. The retention times of amlodipine besylate and indapamide were 5.9 min and 3.6 min, respectively. The proposed method was validated with respect to linearity, accuracy, precision, and robustness. The method was successfully applied to the estimation of amlodipine besylate and indapamide in combined tablet dosage forms.

Keywords: Amlodipine; Indapamide; RP-HPLC; Tablet; Validation.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
Structure of amlodipine besylate (AML) and indapamide (IND)
Fig. 2
Fig. 2
HPLC chromatogram of IND (RT 3.6 min) and AML (RT 5.9 min), using 0.02 M KH2PO4-methanol (30+70, v/v) total pH adjusted to 3 using O-phosphoric acid as mobile phase
See this image and copyright information in PMC

References

    1. O’Neil MJ. The Merck Index. 14th Ed. Merck & Co. Inc.; Whitehouse Station, NJ: 2006.
    1. Anthony CM, Osselton MD. Clarke’s analysis of Drugs and poisons in body fluids and post-mortem material. 3rd edition. Vol. 2. Pharmaceutical press; 2004. p. 629.p. 1132.
    1. Indian Pharmacopoeia 2007-Govt. of India, The Indian pharmacopoeia commission. Ghaziabad: Ministry of Health & Family welfare;
    1. Sharma BK. Instrumental Methods of Chemical Analysis. 20th edition. GOEL Publishing House; 2005.
    1. Q2 (R1), Text on Validation of Analytical Procedures. International Conference on Harmonization (ICH); Geneva, Switzerland. 2005.

LinkOut - more resources