Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2012 Dec;50(12):4100-2.
doi: 10.1128/JCM.02180-12. Epub 2012 Sep 26.

Two techniques for simultaneous identification of Plasmodium ovale curtisi and Plasmodium ovale wallikeri by use of the small-subunit rRNA gene

Affiliations

Two techniques for simultaneous identification of Plasmodium ovale curtisi and Plasmodium ovale wallikeri by use of the small-subunit rRNA gene

Hans-Peter Fuehrer et al. J Clin Microbiol. 2012 Dec.

Abstract

The primers traditionally used to detect Plasmodium ovale infections are known for not binding all P. ovale parasites within the small-subunit rRNA gene when used alone. We describe a simple, cost- and time-efficient multiplex nested PCR and a nested PCR using a novel set of primers for the simultaneous detection of P. ovale curtisi and P. ovale wallikeri.

PubMed Disclaimer

References

    1. Calderaro A, et al. 2007. Genetic polymorphisms influence Plasmodium ovale PCR detection accuracy. J. Clin. Microbiol. 45:1624–1627 - PMC - PubMed
    1. Cox-Singh J, Mahayet S, Abdullah MS, Singh B. 1997. Increased sensitivity of malaria detection by nested polymerase chain reaction using simple sampling and DNA extraction. Int. J. Parasitol. 27:1575–1577 - PubMed
    1. Fuehrer HP, et al. 2010. Indigenous Plasmodium ovale malaria in Bangladesh. Am. J. Trop. Med. Hyg. 83:75–78 - PMC - PubMed
    1. Fuehrer HP, et al. 2011. Novel nested direct PCR technique for malaria diagnosis using filter paper samples. J. Clin. Microbiol. 49:1628–1630 - PMC - PubMed
    1. Fuehrer HP, et al. 2012. Plasmodium ovale in Bangladesh: genetic diversity and the first known evidence of the sympatric distribution of Plasmodium ovale curtisi and Plasmodium ovale wallikeri in southern Asia. Int. J. Parasitol. 42:693–699 - PubMed

Publication types

MeSH terms