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. 2012 Sep;67(9):1077-85.
doi: 10.6061/clinics/2012(09)16.

The effects of alpha-tocopherol supplementation on fracture healing in a postmenopausal osteoporotic rat model

Sharlina Mohamad  1 , Ahmad Nazrun Shuid, Norazlina Mohamed, Fazalina Mohd Fadzilah, Sabarul Afian Mokhtar, Shahrum Abdullah, Faizah Othman, Farihah Suhaimi, Norliza Muhammad, Ima Nirwana Soelaiman
Affiliations

The effects of alpha-tocopherol supplementation on fracture healing in a postmenopausal osteoporotic rat model

Sharlina Mohamad et al. Clinics (Sao Paulo). 2012 Sep.

Abstract

Objective: Osteoporosis increases the risk of bone fractures and may impair fracture healing. The aim of this study was to investigate whether alpha-tocopherol can improve the late-phase fracture healing of osteoporotic bones in ovariectomized rats.

Method: In total, 24 female Sprague-Dawley rats were divided into three groups. The first group was sham-operated, and the other two groups were ovariectomized. After two months, the right femora of the rats were fractured under anesthesia and internally repaired with K-wires. The sham-operated and ovariectomized control rat groups were administered olive oil (a vehicle), whereas 60 mg/kg of alpha-tocopherol was administered via oral gavage to the alpha-tocopherol group for six days per week over the course of 8 weeks. The rats were sacrificed, and the femora were dissected out. Computed tomography scans and X-rays were performed to assess fracture healing and callus staging, followed by the assessment of callus strengths through the biomechanical testing of the bones.

Results: Significantly higher callus volume and callus staging were observed in the ovariectomized control group compared with the sham-operated and alpha-tocopherol groups. The ovariectomized control group also had significantly lower fracture healing scores than the sham-operated group. There were no differences between the alpha-tocopherol and sham-operated groups with respect to the above parameters. The healed femora of the ovariectomized control group demonstrated significantly lower load and strain parameters than the healed femora of the sham-operated group. Alpha-tocopherol supplementation was not able to restore these biomechanical properties.

Conclusion: Alpha-tocopherol supplementation appeared to promote bone fracture healing in osteoporotic rats but failed to restore the strength of the fractured bone.

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Conflict of interest statement

No potential conflict of interest was reported.

Figures

Figure 1
Figure 1
(A) An representative X-ray image used to confirm successful K-wire insertion and that the fractures were in the mid-diaphyseal region. (B) Axial view of healed fractured femora from CT scan images. (I) SO group; (II) OVXC group; (III) ATF group.
Figure 2
Figure 2
X-ray images of a healed fractured femur from each group. (A) SO group; (B) OVXC group; (C) ATF group.
Figure 3
Figure 3
(A) The bar chart indicates the axial callus volumes, which were measured and calculated using CT scanning. (B) The bar chart indicates the fracture healing stages obtained from the radiological scoring of X-ray images. (C) The bar chart indicates the callus staging scores obtained from the radiological scoring of X-ray images. SO: sham-operated group; OVXC: ovariectomized control group; ATF: ovariectomized + ATF-supplemented group; Values with the same alphabetical symbol are significantly different at p<0.05.
Figure 4
Figure 4
(A) The bar chart illustrates the stress parameters derived from bone biomechanical testing. (B) The bar chart indicates the strain parameters derived from bone biomechanical testing. (C) The bar chart illustrates the load parameters derived from bone biomechanical testing. (D) The bar chart indicates the Young's modulus values derived from bone biomechanical testing. SO: sham-operated group; OVXC: ovariectomized control group; ATF: ovariectomized + ATF-supplemented group; Values with the same alphabetical symbol are significantly different p<0.05.
See this image and copyright information in PMC

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