Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2012 Jul-Aug;20(4):449-54.
doi: 10.1590/s1678-77572012000400010.

Dentine microhardness after different methods for detection and removal of carious dentine tissue

Fernanda Brandão Mollica  1 , Carlos Rocha Gomes Torres, Sergio Eduardo de Paiva Gonçalves, Maria Nadir Gasparoto Mancini
Affiliations

Dentine microhardness after different methods for detection and removal of carious dentine tissue

Fernanda Brandão Mollica et al. J Appl Oral Sci. 2012 Jul-Aug.

Abstract

There are several methods for identifying carious dentinal tissue aiming to avoid removal of healthy dentinal tissue.

Objectives: The purpose of this study was to test different methods for the detection of carious dentinal tissue regarding the amount of carious tissue removed and the remaining dentin microhardness after caries removal.

Material and methods: The dentin surfaces of 20 bovine teeth were exposed and half of the surface was protected with nail polish. Cariogenic challenge was performed by immersion in a demineralizing solution for 14 days. After transverse cross-section of the crown, the specimens were divided into four groups (n=10), according to the method used to identify and remove the carious tissue: "Papacárie", Caries-detector dye, DIAGNOdent and Tactile method. After caries removal, the cross-sectional surface was included in acrylic resin and polished. In a microhardness tester, the removed dentin thickness and the Vickers microhardness of the following regions were evaluated: remaining dentin after caries removal and superficial and deep healthy dentin.

Results: ANOVA and Tukey's test (α=0.05) were performed, except for DIAGNOdent, which did not detect the presence of caries. Results for removed dentin thickness were: "Papacárie" (424.7 ± 105.0; a), Caries-detector dye (370.5 ± 78.3; ab), Tactile method (322.8 ± 51.5; bc). Results for the remaining dentin microhardness were: "Papacárie" (42.2 ± 10.5; bc), Caries-detector dye (44.6 ± 11.8; abc), Tactile method (24.3 ± 9.0; d).

Conclusions: DIAGNOdent did not detect the presence of carious tissue; Tactile method and "Papacárie" resulted in the least and the most dentinal thickness removal, respectively; Tactile method differed significantly from "Papacárie" and Caries-detector dye in terms of the remaining dentin microhardness, and Tactile method was the one which presented the lowest microhardness values.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Scheme for the vestibular dentin exposed and embedded in acrylic resin. A- surface submitted to cariogenic challenge (sound dentin); B- surface covered with nail varnish (healthy dentin)
Figure 3
Figure 3
Points and regions of microhardness assessment in dentin. A- remaining sound dentin; B- superficial healthy dentin; C- deep healthy dentin; Dthickness of dentin removed
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Anderson MH, Loesche WJ, Charbeneau GT. Bacteriologic study of a basic fuchsin caries disclosing dye. J Prosthet Dent. 1985;54:51–55. - PubMed
    1. Angker L, Nockolds C, Swain MV, Kilpatrick N. Correlating the mechanical properties to the mineral content of carious dentine - a comparative study using an ultra-micro-indentation system (UMIS) and SEM-BSE signals. Arch Oral Bio. 2004;49:369–378. - PubMed
    1. Banerjee A, Sherriff M, Kidd EA, Watson TF. A confocal microscopic study relating the autofluorescence of carious dentine to its microhardness. Br Dent J. 1999;187:206–210. - PubMed
    1. Craig RG, Gehring PE, Peyton FA. Relation of structure to the microhardness of human dentin. J Dent Res. 1959;38:624–630. - PubMed
    1. Feagin F, Patel PR, Koulourides T, Pigman W. Study of the effect of calcium, phosphate, fluoride and hydrogen ion concentrations on the remineralization of partially demineralized human and bovine enamel surfaces. Arch Oral Bio. 1971;16:535–548. - PubMed