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Comparative Study
. 2012 Sep 27;17(39):20285.
doi: 10.2807/ese.17.39.20285-en.

Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction

V M Corman  1 , I Eckerle, T Bleicker, A Zaki, O Landt, M Eschbach-Bludau, S van Boheemen, R Gopal, M Ballhause, T M Bestebroer, D Muth, M A Müller, J F Drexler, M Zambon, A D Osterhaus, R M Fouchier, C Drosten
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Free article
Comparative Study

Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction

V M Corman et al. Euro Surveill. .
Free article

Erratum in

  • Euro Surveill. 2012;17(40): pii/20288

Abstract

We present two real-time reverse-transcription polymerase chain reaction assays for a novel human coronavirus (CoV), targeting regions upstream of the E gene (upE) or within open reading frame (ORF)1b, respectively. Sensitivity for upE is 3.4 copies per reaction (95% confidence interval (CI): 2.5–6.9 copies) or 291 copies/mL of sample. No cross-reactivity was observed with coronaviruses OC43, NL63, 229E, SARS-CoV, nor with 92 clinical specimens containing common human respiratory viruses. We recommend using upE for screening and ORF1b for confirmation.

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