Visualizing Ca(2+) signatures in plants

Abstract

Ca(2+) is a key player in an astonishing variety of plant signal transduction pathways where transient, spiking or oscillatory changes in cytosolic Ca(2+) levels help to couple environmental or developmental cues to appropriate cellular responses. Understanding whether and how much Ca(2+) signaling contributes to defining stimulus-response specificity has long been a challenge, but recent work has provided strong evidence that specific information can indeed be encoded in the spatiotemporal characteristics of Ca(2+) signals. Identification of the Ca(2+) binding proteins that transduce Ca(2+) signals by regulating downstream effector proteins has revealed a complex network of Ca(2+) sensor families, of which some members show distinct patterns of expression and subcellular localization. By utilizing genetically encoded fluorescent Ca(2+) probes to monitor Ca(2+) changes at high spatiotemporal resolution, it is now possible to explore whether such spatial heterogeneities in Ca(2+) sensor distribution are coordinated with subcellular microdomains of Ca(2+) signaling. Such visualization of Ca(2+) signaling will also help to address which cellular compartments and transporters contribute to mobilizing and sequestering Ca(2+) and thus define stimulus-specific Ca(2+) signatures.