Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2001:Chapter 12:Unit12.4.
doi: 10.1002/0471140856.tx1204s04.

Isolation of neonatal rat cortical astrocytes for primary cultures

J W Allen  1 , L A Mutkus, M Aschner
Affiliations

Isolation of neonatal rat cortical astrocytes for primary cultures

J W Allen et al. Curr Protoc Toxicol. 2001.

Abstract

There is increasing interest in the role of astrocytes as mediators of neurotoxicity. This unit describes a method for preparing astrocyte cultures of greater than 95% purity by enzymatic dissociation from neonatal rat brain. These preparations have both high yield and high viability.

PubMed Disclaimer

LinkOut - more resources