doi: 10.1093/nar/7.8.2199.
Synthesis of the human insulin gene. Part II. Further improvements in the modified phosphotriester method and the synthesis of seventeen deoxyribooligonucleotide fragments constituting human insulin chains B and mini-CDNA
- PMID: 230464
- PMCID: PMC342380
- DOI: 10.1093/nar/7.8.2199
Item in Clipboard
Synthesis of the human insulin gene. Part II. Further improvements in the modified phosphotriester method and the synthesis of seventeen deoxyribooligonucleotide fragments constituting human insulin chains B and mini-CDNA
Nucleic Acids Res.
.
Free PMC article
Abstract
The purification of protected deoxyribooligonucleotides containing phosphotriester internucleotidic linkages has been improved by developing a deactivated silica gel chromatographic technique. The efficiency of this technique as applied in the modified phosphotriester approach has been demonstrated in the rapid synthesis of seventeen pure fragments constituting the sequence of human insulin B and mini-C DNA. The sequence of each oligomer was confirmed by the two-dimensional mobility shift method of fingerprinting.
Similar articles
-
Synthesis of the human insulin gene. Part III. Chemical synthesis of 5'-phosphomonoester group containing deoxyribooligonucleotides by the modified phosphotriester method. Its application in the synthesis of seventeen fragments constituting human insulin C-chain DNA.Nucleic Acids Res. 1980 Dec 11;8(23):5753-65. doi: 10.1093/nar/8.23.5753. Nucleic Acids Res. 1980. PMID: 7008029 Free PMC article.
-
Synthesis of human insulin gene. Part I. Development of reversed-phase chromatography in the modified triester method. Its application in the rapid and efficient synthesis of eight deoxyribooligonucleotides fragments constituting human insulin A DNA.Nucleic Acids Res. 1979 Apr;6(4):1371-85. doi: 10.1093/nar/6.4.1371. Nucleic Acids Res. 1979. PMID: 450699 Free PMC article.
-
Arylsulfonyltetrazoles, new coupling reagents and further improvements in the triester method for the synthesis of deoxyribooligonucleotides.Nucleic Acids Res. 1977 Feb;4(2):353-71. doi: 10.1093/nar/4.2.353. Nucleic Acids Res. 1977. PMID: 190591 Free PMC article.
-
New effective method for the synthesis of oligonucleotides via phosphotriester intermediates.Nucleic Acids Res. 1982 Nov 11;10(21):6675-94. doi: 10.1093/nar/10.21.6675. Nucleic Acids Res. 1982. PMID: 7177853 Free PMC article.
-
Protecting groups in oligonucleotide synthesis.Methods Mol Biol. 1994;26:1-71. doi: 10.1007/978-1-59259-513-6_1. Methods Mol Biol. 1994. PMID: 7508800 Review. No abstract available.
Cited by
-
Synthesis of the human insulin gene. Part III. Chemical synthesis of 5'-phosphomonoester group containing deoxyribooligonucleotides by the modified phosphotriester method. Its application in the synthesis of seventeen fragments constituting human insulin C-chain DNA.Nucleic Acids Res. 1980 Dec 11;8(23):5753-65. doi: 10.1093/nar/8.23.5753. Nucleic Acids Res. 1980. PMID: 7008029 Free PMC article.
-
A study of the efficiency and the problem of sulfonation of several condensing reagents and their mechanisms for the chemical synthesis of deoxyoligoribonucleotides.Nucleic Acids Res. 1980 Nov 25;8(22):5445-59. doi: 10.1093/nar/8.22.5445. Nucleic Acids Res. 1980. PMID: 6894029 Free PMC article.
-
Solid phase phosphotriester synthesis of large oligodeoxyribonucleotides on a polyamide support.Nucleic Acids Res. 1980 Nov 25;8(22):5193-205. doi: 10.1093/nar/8.22.5193. Nucleic Acids Res. 1980. PMID: 7465412 Free PMC article.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
