doi: 10.1073/pnas.76.12.6260.
Evidence that the avian sarcoma virus transforming gene product is a cyclic AMP-independent protein kinase
- PMID: 230504
- PMCID: PMC411843
- DOI: 10.1073/pnas.76.12.6260
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Evidence that the avian sarcoma virus transforming gene product is a cyclic AMP-independent protein kinase
Proc Natl Acad Sci U S A.
1979 Dec.
Abstract
The avian sarcoma virus transforming gene product pp60src has been partially purified by using ion exchange or immunoaffinity chromatography. These preparations contain a cyclic AMP-independent protein kinase activity capable of transferring radiolabel from [gamma-32P]ATP to immune rabbit IgG, casein, and the heavy chain purified from normal rabbit IgG. The casein kinase activity is specifically inhibited by anti-pp60src IgG. Comparison of the pp60src-protein kinase isolated from cells transformed by a wild-type ASV or by an ASV temperature-sensitive transformation mutant revealed that the latter product had a half-life of 3 min at 41 degrees C whereas that of the wild-type product was 20 min.
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