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. 2013 Feb;29(2):281-8.
doi: 10.1007/s11274-012-1181-4. Epub 2012 Sep 30.

Expression of 4kD scorpion defensin and its in vitro synergistic activity with conventional antibiotics

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Expression of 4kD scorpion defensin and its in vitro synergistic activity with conventional antibiotics

Mingfu Niu et al. World J Microbiol Biotechnol. 2013 Feb.

Abstract

The 4kD scorpion defensin (SD) is a potent disulfide-linked peptide. In this study, we expressed it in methylotrophic yeast Pichia pastoris and purified it using Ni-NTA His Bind Resin. We investigated its in vitro antibacterial activity and effect in combination with several conventional antibiotics. We first examined its antibacterial activity towards several Gram-positive and Gram-negative bacteria. Then we used the broth microdilution method to test drugs alone and in combination and used the fractional inhibitory concentration (FIC index) to classify the drug interactions. Our study showed the expressed SD peptide has antibacterial activity against Salmonella typhimurium, E. coli and S. aureus etc. Synergy or additive interaction was observed between SD and Norfloxacin, Polymyxin B and Ampicillin. Cell growth tests showed that combination of SD and Norfloxacin can improve their activity against bacteria. This result maybe permit lower using of the conventional antibiotic agents more effectively and safely.

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References

    1. Biotechnol Appl Biochem. 2007 May;47(Pt 1):1-9 - PubMed
    1. Peptides. 2010 Nov;31(11):1995-2002 - PubMed
    1. Clin Microbiol Rev. 2006 Jul;19(3):491-511 - PubMed
    1. Curr Pharm Des. 2011;17(25):2719-31 - PubMed
    1. PLoS Pathog. 2010 Oct 28;6(10):e1001067 - PubMed

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