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. 2012:5:213-9.
doi: 10.2147/OTT.S36017. Epub 2012 Sep 21.

Upregulation of microRNA-100 predicts poor prognosis in patients with pediatric acute myeloid leukemia

Jin Bai  1 , Aiping Guo, Ze Hong, Wenxia Kuai
Affiliations

Upregulation of microRNA-100 predicts poor prognosis in patients with pediatric acute myeloid leukemia

Jin Bai et al. Onco Targets Ther. 2012.

Abstract

Objective: MicroRNA-100 (miR-100), a small noncoding RNA molecule, acts as a tumor suppressor or an oncogene in different cancers. The aberrant expression of this microRNA has been demonstrated as a frequent event in adult patients with acute myeloid leukemia (AML), but little is known for pediatric AML. The aim of this study was to investigate the expression and clinical significance of miR-100 in pediatric AML.

Methods: The expression levels of miR-100 in bone marrow mononuclear cells were detected by real-time quantitative polymerase chain reaction in a cohort of 106 patients with de novo pediatric AML. The prognostic values of miR-100 in pediatric AML were also analyzed.

Results: Compared with normal controls, upregulation of miR-100 in the bone marrow of pediatric AML patients with statistically significant differences (P < 0.001) was found. The expression levels of miR-100 were found to be significantly higher in pediatric AML patients with extramedullary disease, with the French-American-British classification subtype M7, and with unfavorable day 7 response to induction chemotherapy (P = 0.008, 0.001 and 0.01, respectively). Moreover, both univariate and multivariate analyses revealed that miR-100 upregulation was associated with poorer relapse-free and overall survival in pediatric AML patients.

Conclusion: This is the first report demonstrating the upregulation of miR-100 in pediatric AML, and its association with poor relapse-free and overall survival. These results suggest that miR-100 upregulation may be used as an unfavorable prognostic marker in pediatric AML.

Keywords: microRNA-100; pediatric acute myeloid leukemia; prognosis; real-time quantitative PCR.

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Figures

Figure 1
Figure 1
MicroRNA-100 (miR-100) expression in 106 pediatric acute myeloid leukemia (AML) patients and normal controls. Notes: After normalization to RNU6B expression levels, the expression level of miR-100 in the bone marrow of 106 pediatric AML patients (mean ± standard deviation [SD]: 10.8 ± 1.9) was significantly higher than that of normal controls (mean ± SD: 2.6 ± 0.7, P < 0.001). P-values were calculated using the Mann–Whitney U test.
Figure 2
Figure 2
Kaplan–Meier curves of relapse-free survival (A) and overall survival (B) of pediatric patients with newly diagnosed acute myeloid leukemia (AML) stratified by the level of microRNA-100 (miR-100) expression. Notes: High miR-100 expression was associated with shorter relapse-free (P = 0.008) and overall (P = 0.006) survival in pediatric AML patients.
See this image and copyright information in PMC

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