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. 2012 Nov;9(11):1081-3.
doi: 10.1038/nmeth.2209. Epub 2012 Oct 14.

Automated velocity mapping of migrating cell populations (AVeMap)

Maxime Deforet  1 , Maria Carla Parrini, Laurence Petitjean, Marco Biondini, Axel Buguin, Jacques Camonis, Pascal Silberzan
Affiliations

Automated velocity mapping of migrating cell populations (AVeMap)

Maxime Deforet et al. Nat Methods. 2012 Nov.

Abstract

Characterizing the migration of a population of cells remains laborious and somewhat subjective. Advances in genetics and robotics allow researchers to perform many experiments in parallel, but analyzing the large sets of data remains a bottleneck. Here we describe a rapid, fully automated correlation-based method for cell migration analysis, compatible with standard video microscopy. This method allows for the computation of quantitative migration parameters via an extensive dynamic mapping of cell displacements.

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References

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