Genetic characterization of Yug Bogdanovac virus

Abstract

We present pyrosequencing data and phylogenetic analysis for the full genome of Yug Bogdanovac virus (YBV), a member of the Vesicular stomatitis virus serogroup of the Rhabdoviridae isolated from a pool of Phlebotomus perfiliewi sandflies collected in Serbia in 1976. YBV shows very low nucleotide identities to other members of the Vesicular stomatitis virus serogroup and does not contain a reading frame for C'/C proteins.

Fig. 1

a YBV genome: The non-segmented negative strand 11.2 kb RNA genome sequentially encodes 5 transcription units for the nucleoprotein (N), phosphoprotein (P), matrix protein (M), glycoprotein (G), and the RNA-dependent RNA-Polymerase (L) each followed by a conserved polyadenylation signal (PA) and a short intergenic region. The amino acid sizes of the putative encoded proteins are indicated. b Neighbor-joining phylogenetic analysis of full genome nucleotide sequences using ClustalW and a 1,000-fold bootstrap approach rooted to the sequence of RABV (FJ712195) and collapsed VSV subtree. Bootstrap values are given in percent. YBV (JF911700), CHDV (GU212856, GU212858), ISFV (AJ810084), VSV (EF197793, EU849003, AF473864, AF473865, AF473866, J02428, NC_001560 (all VSIV)), EU373657 (COCV-Ind2), EU373658 (VSAV-Ind3), and RABV (FJ712195). c Alignment of the 3′-leader (YBV 3′ Le) and the positive-sense complement of the 5′-trailer (YBV 5′ TrC) region of YBV. Residues that match the consensus are shaded gray