Pure and mixed lipid black foam films as models of membrane fusion

Abstract

Black foam films (BFF) from water solutions of the phospholipid dilauroyl lecithin (DLL) with admixtures of palmitoyl lysolecithin (Lyso) were formed. Microscopic BFF were studied by the method of Scheludko and Exerowa. The formation probability for BFF and the BFF lifetime in a black state before film rupture were measured as functions of the film composition. At a fixed overal lipid concentration it was shown that an increased percentage of Lyso exponentially increased the lifetime of the film up to the CMC of Lyso. This stabilizing Lyso effect nicely corresponds with its stabilizing action on the waiting time for fusion of two contacting black lipid membranes (BLM), as found by Chernomordik et al. In contrast, Lyso is known to destabilize a single BLM. In this way we have found experimental proof of our earlier prediction that Lyso should have opposite effects on the lifetimes of BLM and BFF. In addition, we have shown for the first time that foam films made of lipids are a convenient model for monolayer membrane fusion studies. This model is characterized by its simplicity and experimental reliability and provides a means for quick screening of the fusogenic capacity of various amphiphilic and hydrophilic admixtures.