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. 2012:517:303-24.
doi: 10.1016/B978-0-12-404634-4.00015-2.

Toward awakening cryptic secondary metabolite gene clusters in filamentous fungi

Fang Yun Lim  1 , James F Sanchez, Clay C C Wang, Nancy P Keller
Affiliations

Toward awakening cryptic secondary metabolite gene clusters in filamentous fungi

Fang Yun Lim et al. Methods Enzymol. 2012.

Abstract

Mining for novel natural compounds is of eminent importance owing to the continuous need for new pharmaceuticals. Filamentous fungi are historically known to harbor the genetic capacity for an arsenal of natural compounds, both beneficial and detrimental to humans. The majority of these metabolites are still cryptic or silent under standard laboratory culture conditions. Mining for these cryptic natural products can be an excellent source for identifying new compound classes. Capitalizing on the current knowledge on how secondary metabolite gene clusters are regulated has allowed the research community to unlock many hidden fungal treasures, as described in this chapter.

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Figures

Figure 15.1
Figure 15.1
Design of transformation cassette: (A) whole deletion of ORF from GOI, (B) internal deletion of ORF from GOI, (C) insertion of selection marker disrupting the ORF of GOI, and (D) native promoter replacement with either a constitutive or inducible promoter.
Figure 15.2
Figure 15.2
Double-joint PCR reaction setup and cycling conditions.
See this image and copyright information in PMC

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