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Randomized Controlled Trial
. 2012 Nov 1;17(6):e956-61.
doi: 10.4317/medoral.18843.

Cytological changes in the oral mucosa after use of a mouth rinse with alcohol. A prospective double blind control study

Jose V Bagan  1 , Francisco Vera-Sempere, Cristina Marzal, Ana Pellín-Carcelén, Ezequiel Martí-Bonmatí, Leticia Bagan
Affiliations
Randomized Controlled Trial

Cytological changes in the oral mucosa after use of a mouth rinse with alcohol. A prospective double blind control study

Jose V Bagan et al. Med Oral Patol Oral Cir Bucal. .

Abstract

Aim: The aim of this preliminary study was to detect cytological changes in the oral mucosa after using a mouth wash with alcohol.

Material and methods: A prospective double-blind, controlled study was performed, for 6 months. Group 1 consisted of 30 subjects who used a mouth rinse with 26.9% of alcohol [Listerine] and Group 2 consisted of 30 subjects who used a mouth rinse with the same ingredients but with no alcohol. We obtained three cytological samples from the oral mucosa. The presence of cytological atypia, binucleation and karyorrhesis, and type of cells were studied. We also used a fluorescent in situ hybridization technique (FISH) in 15 samples in each group, for the micronucleus.

Results: We found no clinical mucosal alteration after using the mouth wash at the end of the study in either group. We observed no cytological differences between the groups at the end of the study (p>0.05). Regarding the study of the micronucleus by FISH, we observed no significant difference between the groups (p>0.05).

Conclusions: Our results showed no cytological alteration in patients using a mouth rinse with alcohol, but these findings should be considered preliminary results, to be confirmed in a greater sample of patients.

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Conflict of interest statement

Conflict of interest statement: The authors have declared that no conflict of interest exist.

Figures

Figure 1
Figure 1
Micronucleus in oral squamous cell observed by FISH technique: A) DAPI stain, B) FISH using the “All Human Centromere Probe” revealed green centromeric signals in both the nucleus and micronucleus (FISH, 1000×).
See this image and copyright information in PMC

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