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Comparative Study
. 2012:2012:928159.
doi: 10.1155/2012/928159. Epub 2012 Oct 3.

The inhibitory effect of Prunella vulgaris L. on aldose reductase and protein glycation

Hong Mei Li  1 , Jin Kyu Kim, Jai Man Jang, Sang Oh Kwon, Cheng Bi Cui, Soon Sung Lim
Affiliations
Comparative Study

The inhibitory effect of Prunella vulgaris L. on aldose reductase and protein glycation

Hong Mei Li et al. J Biomed Biotechnol. 2012.

Abstract

To evaluate the aldose reductase (AR) enzyme inhibitory ability of Prunella vulgaris L. extract, six compounds were isolated and tested for their effects. The components were subjected to in vitro bioassays to investigate their inhibitory assays using rat lens aldose reductase (rAR) and human recombinant AR (rhAR). Among them, caffeic acid ethylene ester showed the potent inhibition, with the IC(50) values of rAR and rhAR at 3.2 ± 0.55 μM and 12.58 ± 0.32 μM, respectively. In the kinetic analyses using Lineweaver-Burk plots of 1/velocity and 1/concentration of substrate, this compound showed noncompetitive inhibition against rhAR. Furthermore, it inhibited galactitol formation in a rat lens incubated with a high concentration of galactose. Also it has antioxidative as well as advanced glycation end products (AGEs) inhibitory effects. As a result, this compound could be offered as a leading compound for further study as a new natural products drug for diabetic complications.

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Figures

Figure 1
Figure 1
Structures of compounds isolated from P. vulgaris L.
Figure 2
Figure 2
Lineweaver-Burk plots showing the reciprocal of the velocity (1/v) of recombinant human aldose reductase versus the reciprocal of the substrate concentration (1/s) with dl-glyceraldehyde as the substrate concentration of 0.1 to 1 mM; rosmarinic acid (4), caffeic acid ethylene ester (5).
See this image and copyright information in PMC

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