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. 2013 Jan 4;112(1):17-22.
doi: 10.1161/CIRCRESAHA.112.281048. Epub 2012 Oct 23.

Smooth muscle cell plasticity: fact or fiction?

Anh T Nguyen  1 , Delphine Gomez, Robert D Bell, Julie H Campbell, Alexander W Clowes, Giulio Gabbiani, Cecilia M Giachelli, Michael S Parmacek, Elaine W Raines, Nancy J Rusch, Mei Y Speer, Michael Sturek, Johan Thyberg, Dwight A Towler, Mary C Weiser-Evans, Chen Yan, Joseph M Miano, Gary K Owens
Affiliations

Smooth muscle cell plasticity: fact or fiction?

Anh T Nguyen et al. Circ Res. .
No abstract available

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Figures

Figure 1
Figure 1
Synthetic smooth muscles contribute to vascular remodeling following wire injury. (a-f) Lineage-tracing data at 5 days post-carotid wire injury using a non-inducible SM-MHC-Cre/eGFP floxed-stop Rosa GFP mouse, adapted from Tang et al.7. Following wire injury, no SMC can be detected in the media (d), precluding the ability to analyze the role of SMC phenotypic modulation. The media is replaced by cells expressing S100b (e). (g-l) Lineage-tracing data at 7 days post-femoral artery wire injury using a tamoxifen-inducible SM MHC-CreERT2/Rosa26-floxStop βGal mouse, adapted from Nemenoff et al. . Following wire injury, intimal bGal+SMaA+ SMC (arrows) and bGal+SMaA-phenotypically modulated SMC (open arrowheads) are observed, including BrdU+ LacZ+ proliferating cells (see Fig. 1c of Nemenoff et al. supporting the role of synthetic SMC in vascular remodeling and neointima formation. Lines delineate the arterial media. (m-t) Lineage tracing data at 7 days post-carotid wire injury using SM-MHC-LacZ, SM22a-LacZ, or SM22agc-LacZ (mutated G/C repressor) reporter mice, adapted from Regan et al.. Following wire injury, SMC de-differentiate as evidenced by no positive staining in SM-MHC-LacZ mice SM22a-LacZ. Medial and intimal phenotypically modulated SMC are identified by LacZ+ staining post-injury in the SM22agc-LacZ (mutated G/C repressor) reporter mice, further supporting the role of synthetic SMC in vascular remodeling and neointima formation. Arrows denote internal elastic lamina and arrowheads denote external elastic lamina.
Figure 2
Figure 2
SM MHC+ SMC replicate following arterial injury. (A) Section of femoral artery of SM MHC-CreERT2/Rosa26-floxStop-βGal reporter mouse 7 days following wire injury [data provided by the laboratory of Dr. Mary C. Weiser-Evans] [see Nemenoff et al.11 for additional methodological details]. Arrow heads denote internal elastic lamina and arrows indicate proliferating BrdU+ SMC. (B, C) 10 μm thick reconstructed Z-stacked confocal microscopy of twenty 0.5 μm images from a wildtype mouse carotid artery 7 days following ligation injury [unpublished data provided by the laboratory of Dr. Joseph M. Miano]. Most medial cells are SM MHC positive (green) with many exhibiting clear Ki67 staining (magenta). Nuclei are stained with DAPI. The bar in panel B is 50 μm. Boxed region in panel B is shown at higher magnification in panel C with arrows pointing to obvious SM MHC+/Ki67+ medial SMC and arrowheads indicating weakly stained SM MHC+/Ki67+ cells. The bar in panel C is 10 μm.
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References

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