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Comparative Study
. 2012 Dec;19(12):1972-6.
doi: 10.1128/CVI.00535-12. Epub 2012 Oct 24.

Low sensitivity of NS1 protein tests evidenced during a dengue type 2 virus outbreak in Santos, Brazil, in 2010

Alvina Clara Felix  1 , Camila Malta Romano, Cristiane de Campos Centrone, Célia Lima Rodrigues, Lucy Villas-Boas, Evaldo Stanislau Araújo, Andréia Manso de Matos, Karina Inácio Carvalho, Celina Maria Turchi Martelli, Esper Georges Kallas, Claúdio Sérgio Pannuti, José Eduardo Levi
Affiliations
Comparative Study

Low sensitivity of NS1 protein tests evidenced during a dengue type 2 virus outbreak in Santos, Brazil, in 2010

Alvina Clara Felix et al. Clin Vaccine Immunol. 2012 Dec.

Abstract

In 2010, a large outbreak of dengue occurred in Santos, Brazil. The detection of the NS1 antigen was used for diagnosis in addition to the detection of IgG, IgM, and RNA. A large number of NS1 false-negative results were obtained. A total of 379 RNA-positive samples were selected for thorough evaluation. NS1 was reactive in 37.7% of cases. Most of the cases were characterized as a secondary infection by dengue 2 virus. Sequencing of NS1 positive and negative isolates did not reveal any mutation that could justify the diagnostic failure. Use of existing NS1 tests in the Brazilian population may present a low negative predictive value, and they should be used with caution, preferentially after performing a validation with samples freshly obtained during the ongoing epidemic.

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Figures

Fig 1
Fig 1
Rate (%) of reactivity for NS1 and IgM according to the days since the onset of symptoms for secondary infection (n = 260).
See this image and copyright information in PMC

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