Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2007 Dec;47(4):373-6.
doi: 10.1007/s12088-007-0067-x. Epub 2008 Jan 11.

Detection of human rotavirus in hospitalized diarrheic children in central India

Anupam Lal Kusumakar  1 , Savita, Yashpal Malik, Minakshi, Gaya Prasad
Affiliations

Detection of human rotavirus in hospitalized diarrheic children in central India

Anupam Lal Kusumakar et al. Indian J Microbiol. 2007 Dec.

Abstract

During the present study, group A human rotaviruses were detected among diarrheic children using polyacrylamide gel electrophoresis (PAGE) technique, with a typical RNA migration pattern of 4:2:3:2, suggestive of group A rotavirus. During the study, a total of 46 fecal samples collected from hospitalized children with acute diarrhea as well as children inhabiting nearby animal farms with history of presence of animal rotaviruses on the farms were processed for detection of human rotavirus. Out of 33 diarrheic children, 12 showed presence of rotavirus infection (36.36%), however, none of the children from animal farm areas showed presence of rotavirus. Female children were more susceptible to rotavirus infection (46.15%) than males (30%). Majority of the cases of rotavirus gastroenteritis belonged up to one year of the age, with an incidence of 40.91%. RNA profile of rotaviruses suggested circulation of 5 different electropherotypes in this geographical locale of the country, indicating existence of genomic diversity among human rotaviruses. Majority of the isolates were of long pattern (66.67%), whereas short pattern was detected only in one third of the viruses. This preliminary study emphasizes for further detailed studies on the molecular characterization of rotaviruses circulating in this part of country and their relationship with other human rotavirus strains and animal strains in the country.

Keywords: Diarrhea; Group A human rotavirus; RNA-PAGE; detection; electropherotypes.

PubMed Disclaimer

References

    1. Kapikian A.Z., Chanock R.M. Rotaviruses. In: Fields B.N., Knipe D.M., Howley P.M., editors. Virology. v. 2. Philadelphia: Lippincott-Raven Press; 1996. pp. 1657–1708.
    1. Estes M.K., Graham D.Y., Dimitrov D.H. The molecular epidemiology of rotavirus gastroenteritis. Prog Med Virol. 1984;29:1–22. - PubMed
    1. Blumer C., Roche P., Kirkwood C., Bishop R., Barnes G. Surveillance of viral pathogens in Australia. Commun Dis Intellegence. 2003;27:496–503. - PubMed
    1. Broor S., Husain M., Chatterjee B., Chakraborty A., Seth P. Temporal variation in the distribution of rotavirus electropherotypes in Delhi, India. J Diarrh Dis Res. 1993;11:14–18. - PubMed
    1. Aijaz S., Gowda K., Jagannath H.V., Reddy R.R., Maiya P.P., Ward R.L., Greenberg H.B., Raju M., Babu A., Rao C.D. Epidemiology of symptomatic human rotaviruses in Bangalore and Mysore, India, from 1988 to 1994 as determined by elctropherotye, subgroup and serotype analysis. Arch Virol. 1988;141:1–12. - PubMed

LinkOut - more resources